Induction of caspase-9, biochemical assessment and morphological changes caused by apoptosis in cancer cells treated with goniothalamin extracted from Goniothalamus macrophyllus.

Abstract

Goniothalamin, a natural compound extracted from Goniothalamus sp. belonging to the Annonacae family, possesses anticancer properties towards several tumor cell lines. This study focused on apoptosis induction by goniothalamin (GTN) in the Hela cervical cancer cell line. Cell growth inhibition was measured by MTT assay and the IC50 value of goniothalamin was 3.2 ± 0.72 μg/ml. Morphological changes and biochemical processes associated with apoptosis were evident on phase contrast microscopy and fluorescence microscopy. DNA fragmentation, DNA damage, caspase-9 activation and a large increase in the sub-G1 and S cell cycle phases confirmed the occurrence of apoptosis in a time-dependent manner. It could be concluded that goniothalamin show a promising cytotoxicity effect against cervical cancer cells (Hela) and the cell death mode induced by goniothalamin was apoptosis.

• Authors: Aied Mohammed Alabsi, Rola Ali, Abdul Manaf Ali, Hazlan Harun, Sami Abdo Radman Al-Dubai, Kurubaran Ganasegeran, Mustafa Ahmed Alshagga, Sameer Dirhim Salem, Noor Hayaty Binti Abu Kasim
• Journal: Asian Pacific journal of cancer prevention : APJCP (Asian Pac J Cancer Prev) Vol. 14 Issue 11 Pg. 6273-80 ( 2013) ISSN: 2476-762X [Electronic] Thailand
• PMID: 24377517 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Plant Extracts
• Pyrones
• goniothalamin
• Caspase 9

Topics

• Apoptosis (drug effects)
• Caspase 9 (biosynthesis, metabolism)
• Cell Death (drug effects)
• Cell Line, Tumor
• DNA Damage (drug effects)
• DNA Fragmentation (drug effects)
• Enzyme Induction
• G1 Phase (drug effects)
• Goniothalamus (chemistry)
• HeLa Cells
• Humans
• Plant Extracts (chemistry, pharmacology)
• Pyrones (chemistry, pharmacology)
• S Phase (drug effects)