Abstract | AIMS: MATERIALS & METHODS: The HIV derived peptides uptake in DC and the phenotype of iDC and mDC were studied using Flow Cytometry analysis. Migration of mDC was evaluated by an in vitro chemotaxis assay. Allogenic T-cells proliferative response induced by DC was studied using Flow Cytometry assays. Cytokines production was analysed by Diaclon DIAplex Th1/Th2/ Inflammation kit. RESULTS: All phosphorus dendrimers showed the ability to deliver HIV-derived peptides in DC. The phosphorus dendrimers from second and third generations induced important changes in phenotype. Moreover, the treatment of mDC with the second generation dendrimer and derivated dendriplexes modified cellular migratory properties, altered their capacity to stimulate allogenic naïve T cells in vitro and impeded the production of pro-inflammatory cytokines. CONCLUSIONS:
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Authors | Enrique Vacas-Córdoba, Hugo Bastida, Marjorie Pion, Aurélien Hameau, Maksim Ionov, Maria Bryszewska, Anne Marie Caminade, Jean Pierre Majoral, María-Ángeles Muñoz-Fernández |
Journal | Current medicinal chemistry
(Curr Med Chem)
Vol. 21
Issue 16
Pg. 1898-909
( 2014)
ISSN: 1875-533X [Electronic] United Arab Emirates |
PMID | 24304285
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cytokines
- Dendrimers
- HIV Antigens
- Phosphorus
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Topics |
- Cell Movement
- Cytokines
(metabolism)
- Dendrimers
(chemistry, therapeutic use)
- Dendritic Cells
(immunology, metabolism)
- HIV Antigens
(immunology)
- Humans
- Immunotherapy
- Phosphorus
(chemistry)
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