The control of an infectious
viral disease as
rabies is made easier by rapid and accurate diagnosis. Successful
rabies prophylaxis is dependent upon the active immunization with
vaccine along with passive administration of rabies virus
neutralizing antibodies which together clear the virus before widespread
infection of central nervous system occurs. The present study aimed at the development of
monoclonal antibodies (MAbs) suitable for rabies virus antibody and
antigen detection. For the production of
rabies specific MAbs, immunization of Swiss albino mice with a commercially available
vaccine was done and
Polyethylene glycol mediated fusion of spleenocytes with myeloma cells was performed. The positive clones were selected on the basis of distinct reactivity by cell
Enzyme linked
immunosorbent assay and fluorescence in Indirect Fluorescent antibody test. The positive clones obtained were subjected to single cell cloning by limiting dilution method. The reactive clones were further titrated and employed for virus titration and virus neutralization. The neutralizing activity was evaluated using Fluorescence Activated Cell Sorter technique. Three MAb clones showed a distinct percent inhibition in the presence of positive serum. One of the MAb clone No. 5C3 was relatively more specific in detecting
rabies antibodies and also found suitable for competitive ELISA to assess the antibody level in vaccinated subjects.