Evaluation of the interaction between sodium hypochlorite and several formulations containing chlorhexidine and its effect on the radicular dentin--SEM and push-out bond strength analysis.

Abstract

The aim of the current study was to evaluate the presence of debris and smear layer after endodontic irrigation with different formulations of 2% chlorhexidine gluconate (CHX) and its effects on the push-out bond strength of an epoxy-based sealer on the radicular dentin. One hundred extracted human canines were prepared to F5 instrument and irrigated with 2.5% sodium hypochlorite and 17% ethylenediaminetetraacetic acid. Fifty teeth were divided into five groups (n = 10), according to the final irrigation protocol with different 2% CHX formulations: G1 (control, no final rinse irrigation), G2 (CHX solution), G3 (CHX gel), G4 (Concepsis), and G5 (CHX Plus). In sequence, the specimens were submitted to scanning electron microscopy (SEM) analysis, in the cervical-medium and medium-apical segments, to evaluate the presence of debris and smear layer. The other 50 teeth were treated equally to a SEM study, but with the root canals filled with an epoxy-based endodontic sealer and submitted to a push-out bond strength test, in the cervical, middle, and apical thirds. G2, G3, G4, and G5 provided higher precipitation of the debris and smear layer than G1 (P < 0.05), but these groups were similar to each other (P > 0.05), in both segments. The values obtained in the push out test did not differ between groups, independent of the radicular third (P > 0.05). The CHXs formulations caused precipitation of the debris and smear layer on the radicular dentin, but these residues did not interfere in the push-out bond strength of the epoxy-based sealer.

• Authors: Miriam Graziele Magro, Milton Carlos Kuga, Keli Regina Victorino, Fernando Antonio Vázquez-Garcia, Arturo Javier Aranda-Garcia, Norberto Batista Faria-Junior, Gisele Faria, André Luis Shinohara
• Journal: Microscopy research and technique (Microsc Res Tech) Vol. 77 Issue 1 Pg. 17-22 (Jan 2014) ISSN: 1097-0029 [Electronic] United States
• PMID: 24190651 (Publication Type: Journal Article)
• Copyright: Copyright © 2013 Wiley Periodicals, Inc.

Chemical References

• Anti-Bacterial Agents
• Root Canal Irrigants
• Sodium Hypochlorite
• chlorhexidine gluconate
• Chlorhexidine

Topics

• Aged
• Anti-Bacterial Agents (chemistry)
• Chlorhexidine (analogs & derivatives, chemistry)
• Cuspid (chemistry, ultrastructure)
• Dental Stress Analysis
• Dentin (chemistry, ultrastructure)
• Female
• Humans
• Male
• Microscopy, Electron, Scanning
• Middle Aged
• Peritoneal Lavage (instrumentation)
• Root Canal Irrigants (chemistry)
• Root Canal Preparation (instrumentation)
• Sodium Hypochlorite (chemistry)