Abstract | BACKGROUND: METHOD:
Tumor and colon mucosa tissue were collected at primary intended curative operations in patients according to well-recognized statistical distributions of tumor stages in colorectal cancer. COX-1, COX-2 and EGFR content in tumor and colon mucosa tissue were quantified by western blot and Q-PCR. RESULTS: COX-2 protein appeared as two bands, one at 66 kDa in almost all tumor and mucosa samples and one at 74 kDa in 73% of the tumors and in 23% of the mucosa samples. Tumor COX-2 mRNA was not different from the content in mucosa samples, while COX-2 protein was increased in tumor tissue (p < 0.0003). A correlation between 74 kDa COX-2 protein and COX-2 mRNA occurred in tumor tissue, with significantly increasing COX-2 mRNA across tumor stages. EGFR mRNA content was lower in tumor tissue (p < 0.0001), while EGFR protein was similar in tumor and mucosa samples. COX-2 and EGFR proteins showed a positive correlation in mucosa, while a negative correlation occurred in tumor tissue. Tumor tissue with high COX-2 74 kDa protein lacked EGFR protein. CONCLUSION: Our present results are compatible with the theory that COX-2 and EGFR signalling pathways are inversely related in colorectal cancer tissue. This may explain why combinatorial clinical treatments have been less rewarding.
|
Authors | Annika Gustafsson Asting, Ava Farivar, Britt-Marie Iresjö, Helena Svensson, Bengt Gustavsson, Kent Lundholm |
Journal | BMC cancer
(BMC Cancer)
Vol. 13
Pg. 511
(Oct 30 2013)
ISSN: 1471-2407 [Electronic] England |
PMID | 24171795
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- RNA, Messenger
- Cyclooxygenase 1
- Cyclooxygenase 2
- ErbB Receptors
|
Topics |
- Aged
- Aged, 80 and over
- Biopsy
- Colorectal Neoplasms
(genetics, metabolism, pathology)
- Cyclooxygenase 1
(genetics, metabolism)
- Cyclooxygenase 2
(genetics, metabolism)
- ErbB Receptors
(genetics, metabolism)
- Female
- Humans
- Intestinal Mucosa
(metabolism, pathology)
- Male
- Middle Aged
- Neoplasm Staging
- RNA, Messenger
(genetics, metabolism)
|