Ultrasound bio-microscopy for measurement of coronary artery flow and estimation of infarct size in a mouse model of acute myocardial infarction.

Abstract

Ultrasound bio-microscopy was used to measure hemodynamic changes in the left main coronary artery after myocardial infarction (MI), and its usefulness in estimating infarct size was evaluated. MI was induced by left anterior descending artery ligation. Diastolic peak velocity (Vd), mean flow velocity (Vmean) and the velocity-time integral (VTI) were measured 2 and 6 h after MI. Serum troponin I levels were assayed 2, 6 and 12 h after MI. At 2 h, Vmean and VTI significantly differed between mice that underwent low and high left anterior descending artery ligation; Vd, Vmean and VTI were correlated with infarct size (r = -0.557, -0.693 and -0.672, respectively; all p < 0.01). Infarct size was more strongly correlated with 2-h ultrasound bio-microscopy measurements than with 2-h serum troponin I level. Measurement of coronary artery blood flow by ultrasound bio-microscopy may be useful for early estimation of infarct size in mice.

• Authors: Rui-Juan Su, Jun-Meng Zhang, Rong-Juan Li, Yan Sun, Bo Jiang, Ning Ma, Zhi-An Li, Xiang-Hong Luo, Li Song, Jing-Li Xue, Zheng Wang, Ya Yang
• Journal: Ultrasound in medicine & biology (Ultrasound Med Biol) Vol. 39 Issue 11 Pg. 2044-52 (Nov 2013) ISSN: 1879-291X [Electronic] England
• PMID: 23993171 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved.

Topics

• Animals
• Blood Flow Velocity
• Coronary Circulation
• Disease Models, Animal
• Humans
• Image Interpretation, Computer-Assisted (methods)
• Mice
• Mice, Inbred C57BL
• Microscopy, Acoustic (methods)
• Myocardial Infarction (diagnostic imaging, physiopathology)
• Reproducibility of Results
• Sensitivity and Specificity