Abstract |
Psoriasis is considered a Th17-type autoimmune skin inflammatory disease; however, involvement of an autoantigen-specific TCR has not been established. In this study, we show that psoriasis-like skin inflammation can be induced by autoreactive Th17 cells. We previously developed the desmoglein 3-specific TCR-transgenic (Dsg3H1) mouse, in which CD4⁺ T cells recognize physiological epidermal autoantigen. T cells from Dsg3H1 mice were polarized into Th17 cells in vitro and then adoptively transferred into Rag2⁻/⁻ mice. Dsg3H1-Th17 cells induced severe psoriasis-like skin inflammation within 2 wk after transfer in the tissues in which desmoglein 3 is expressed. Such pathology was not observed when wild-type Th17 cells or Th1-skewed Dsg3H1 T cells were transferred, and it was strongly suppressed by anti- IL-12/23 and anti-IL-17 Abs. Although IFN-γ⁺/IL-17⁺ T cells accumulated in the skin lesions of mice that received Dsg3H1-Th17 cells, IFN-γ-deficient Dsg3H1-Th17 cells were fully pathogenic. These results demonstrate that cutaneous psoriasis-like immunopathology can be developed by epidermis-specific recognition of Th17 cells, which is strictly dependent on IL-17 but not IFN-γ.
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Authors | Shuhei Nishimoto, Hitoshi Kotani, Sanae Tsuruta, Nana Shimizu, Minako Ito, Takashi Shichita, Rimpei Morita, Hayato Takahashi, Masayuki Amagai, Akihiko Yoshimura |
Journal | Journal of immunology (Baltimore, Md. : 1950)
(J Immunol)
Vol. 191
Issue 6
Pg. 3065-72
(Sep 15 2013)
ISSN: 1550-6606 [Electronic] United States |
PMID | 23956432
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Autoantigens
- Receptors, Antigen, T-Cell
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Topics |
- Adoptive Transfer
- Animals
- Autoantigens
(immunology)
- Cell Separation
- Dermatitis
(immunology)
- Disease Models, Animal
- Flow Cytometry
- Immunohistochemistry
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Mice, Transgenic
- Psoriasis
(immunology)
- Real-Time Polymerase Chain Reaction
- Receptors, Antigen, T-Cell
(immunology)
- Reverse Transcriptase Polymerase Chain Reaction
- Skin
(immunology)
- Th17 Cells
(immunology)
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