Serotype-specific anti-Dengue virus NS1 mouse antibodies cross-react with prM and are potentially involved in virus production.

Abstract	Dengue virus (DENV) infection induces a strong B-cell immune response against the viral nonstructural protein 1 (NS1). Anti-NS1 antibodies (Abs) may affect virus production because they coexist with the virus in the patients' blood. The present study examined whether ten mouse monoclonal antibodies (MAbs) raised against NS1 affected production of the DENV-2. Three MAbs, 4C2, 4G11, and 4E5, showed weak neutralizing activity in a focus reduction assay. In addition, two serotype-specific MAbs, 4C2 and 4G11, protected suckling mice from lethal infection with DENV-2. An immunoprecipitation assay with DENV-2 showed that these MAbs, which were specific for the NS1 of DENV-4 and DENV-1, cross-reacted with the DENV-2 pre-membrane (prM) protein, but not with DENV-2 NS1. Interestingly, high concentrations of MAb 4G11 showed antibody-dependent enhancement of DENV-2 infection in human monocyte THP-1 cells. Taken together, these observations suggest that serotype-specific anti-NS1 MAbs are potentially involved in virus production.
Authors	Promsin Masrinoul, Magot Diata Omokoko, Sabar Pambudi, Kazuyoshi Ikuta, Takeshi Kurosu
Journal	Viral immunology (Viral Immunol) Vol. 26 Issue 4 Pg. 250-8 (Aug 2013) ISSN: 1557-8976 [Electronic] United States
PMID	23941673 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Antibodies, Monoclonal Antibodies, Viral NS1 protein, Dengue virus type 2 NS1 protein, dengue-1 virus Viral Envelope Proteins Viral Nonstructural Proteins prM protein, Flavivirus
Topics	Aedes Animals Antibodies, Monoclonal (immunology) Antibodies, Viral (blood, immunology) Cells, Cultured Chlorocebus aethiops Cricetinae Cross Reactions (immunology) Dengue (immunology, prevention & control) Dengue Virus (immunology) HEK293 Cells Humans Mice Mice, Inbred BALB C Vero Cells Viral Envelope Proteins (immunology) Viral Nonstructural Proteins (immunology) Virus Replication (immunology)

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