3-O-Acetyloleanolic acid exhibits anti-angiogenic effects and induces apoptosis in human umbilical vein endothelial cells.

Abstract

3-O-Acetyloleanolic acid, a pentacyclic triterpenoid isolated from cowpea seeds, inhibited proliferation, migration and tube formation of human umbilical vein endothelial cells (HUVECs) in a dose-dependent manner. HUVECs. The induced apoptosis was characterized by detection of cell surface annexin V and sub-G1 populations. The number of cells immunostained with annexin V-fluorescein isothiocyanate increased after treatment with 3-O-acetyloleanolic acid. The sub-G1 cell populations were also increased in treated HUVECs. 3-O-Acetyloleanolic acid induced activation of caspase 3, a critical mediator of apoptosis signaling. It also significantly inhibited angiogenesis in an in vivo Matrigel plug assay. 3-O-Acetyloleanolic acid thus exhibits anti-angiogenic effects and induces apoptosis in HUVECs and the results suggest that it has a potential use for suppression of the tumor growth stimulated by angiogenesis.

• Authors: En-Ji Cui, Jeon Hwang-Bo, Jong-Hwa Park, Nam-In Baek, Jiyoung Kim, Seong Gil Hong, In Sik Chung
• Journal: Biotechnology letters (Biotechnol Lett) Vol. 35 Issue 11 Pg. 1807-15 (Nov 2013) ISSN: 1573-6776 [Electronic] Netherlands
• PMID: 23801119 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Angiogenesis Inhibitors
• Triterpenes
• oleanolic acid 3-acetate

Topics

• Angiogenesis Inhibitors (isolation & purification, metabolism)
• Apoptosis
• Fabaceae (chemistry)
• Human Umbilical Vein Endothelial Cells (drug effects)
• Humans
• Seeds (chemistry)
• Triterpenes (isolation & purification, metabolism)