Substance P enhances HIV-1 infection in human fetal brain cell cultures expressing full-length neurokinin-1 receptor.

Abstract

The associations between the neurokinin-1 receptor (NK-1R), substance P (SP), and HIV-1 were investigated in neurosphere-derived cultures of microglial-depleted human fetal brain cells (HFBC). Full-length NK-1R was identified in HFBC cultures. SP treatment of the HFBC increased intracellular calcium mobilization and decreased electrical impedance, both of which were blocked by the NK-1R antagonist aprepitant. SP treatment of HIV-1-infected HFBC upregulated HIV-1 expression. These data show that human neural cells grown from neurospheres express functional full length NK-1R that is responsive to SP, and that SP enhanced HIV-1 infection in HBFC.

• Authors: Lynnae Schwartz, Sergei V Spitsin, John Meshki, Florin Tuluc, Steven D Douglas, John H Wolfe
• Journal: Journal of neurovirology (J Neurovirol) Vol. 19 Issue 3 Pg. 219-27 (Jun 2013) ISSN: 1538-2443 [Electronic] United States
• PMID: 23765222 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Morpholines
• Neurokinin-1 Receptor Antagonists
• Receptors, Neurokinin-1
• Aprepitant
• Substance P
• Calcium

Topics

• Aprepitant
• Brain (cytology, drug effects, metabolism, virology)
• Calcium (metabolism)
• Electric Impedance
• Fetus
• Gene Expression
• HIV-1 (drug effects, physiology)
• Host-Pathogen Interactions
• Humans
• Ion Transport (drug effects)
• Morpholines (pharmacology)
• Neurokinin-1 Receptor Antagonists (pharmacology)
• Neurons (cytology, drug effects, metabolism, virology)
• Primary Cell Culture
• Receptors, Neurokinin-1 (genetics, metabolism)
• Substance P (antagonists & inhibitors, pharmacology)
• Virus Activation (drug effects)