A novel report of apoptosis in human lung carcinoma cells using selective agonist of D2-like dopamine receptors: a new approach for the treatment of human non-small cell lung cancer.

Abstract

In our previous study, a relationship between low expression of D2-like dopamine receptor genes and non-small cell lung cancer (NSCLC) disease was found. In this new research, by using selective agonist of these receptors, Bromocriptine (BR), we attempted to activate D2-like expression and apoptotic induction in a selective cell line of NSCLC. In addition, the relationship of apoptotic response of human lung carcinoma cells to BR and D2- dopamine receptor genes is investigated. Human lung cancer (QU-DB) cells were treated by five doses of BR at 48 h and cell viability was determined by MTT assay. The gene expression pattern of D2-like dopamine receptor Genes was studied by Real Time PCR. Nuclear morphology of cells was monitored by DAPI flourescent staining then induction of DNA fragmentation by BR was shown in an agarose gel. Finally, the detection and quantification of apoptosis and its differentiation from necrosis was carried out by using Annecxin-V-Fluos Staining. In this study, it is demonstrated that BR inhibited the proliferation of human lung cancer cells and induced apoptosis in them. In addition, the probable relationship between D2-dopamine receptor genes expression and the development of apoptosis was found. In conclusion, BR is responsible for induction of apoptosis in human lung cancer cells and can be used in treatment of these tumoric cells. In addition, normal expression of D2 dopamine receptors was associated with apoptotic effect of BR on these cells.

• Authors: M Sheikhpour, G Ahangari, M Sadeghizadeh, A Deezagi
• Journal: International journal of immunopathology and pharmacology (Int J Immunopathol Pharmacol) 2013 Apr-Jun Vol. 26 Issue 2 Pg. 393-402 ISSN: 0394-6320 [Print] England
• PMID: 23755754 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• DRD2 protein, human
• Dopamine Agonists
• Receptors, Dopamine D2
• Bromocriptine

Topics

• Antineoplastic Agents (pharmacology)
• Apoptosis (drug effects)
• Bromocriptine (pharmacology)
• Carcinoma, Non-Small-Cell Lung (genetics, metabolism, pathology)
• Cell Line, Tumor
• Cell Nucleus Shape (drug effects)
• Cell Proliferation (drug effects)
• Cell Survival (drug effects)
• Dopamine Agonists (pharmacology)
• Dose-Response Relationship, Drug
• Gene Expression Regulation, Neoplastic
• Humans
• Lung Neoplasms (genetics, metabolism, pathology)
• Receptors, Dopamine D2 (agonists, genetics, metabolism)
• Time Factors