Abstract |
Asthma is a Th2-mediated disease that involves Th2 cell and eosinophil migration into the bronchial mucosa which is dependent upon the expression of a specific set of chemokines within the lung. Among them, CCL18 seems to play a key role because of its preferential expression in the lung, and its up-regulation by Th2 cytokines. Here, we show that the optimal naïve T cell and basophil chemotaxis, and basophil histamine release induced by rhCCL18 occurred at a 100 time lower concentration with CHO-derived rhCCL18 than with E. coli-derived rhCCL18. FT-ICR mass spectrometry of the intact chemokines showed that the rhCCL18 produced by CHO cells contained the 2 disulfide bonds Cys10-Cys34 and Cys11-Cys50, in clear contrast to the rhCCL18 derived from E. coli where the Cys10-Cys34 bond was absent. We found that reduction of the Cys10-Cys34 of the CHO-derived rhCCL18 resulted in a shift of its activity, reaching the same level as the E. coli-derived rhCCL18. These results demonstrate that the Cys10-Cys34 disulfide bond is involved in the function of CCL18.
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Authors | Benjamin Legendre, Caroline Tokarski, Ying Chang, Nathalie De Freitas Caires, Hugues Lortat-Jacob, Patricia De Nadaï, Christian Rolando, Catherine Duez, Anne Tsicopoulos, Philippe Lassalle |
Journal | Cytokine
(Cytokine)
Vol. 64
Issue 1
Pg. 463-70
(Oct 2013)
ISSN: 1096-0023 [Electronic] England |
PMID | 23742785
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 Elsevier Ltd. All rights reserved. |
Chemical References |
- CCL18 protein, human
- Chemokines, CC
- Histamine
- Cysteine
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Topics |
- Animals
- Asthma
(immunology, metabolism)
- Basophils
(immunology, metabolism)
- CHO Cells
- Cell Line
- Cell Movement
(immunology)
- Chemokines, CC
(chemistry, genetics, metabolism)
- Cricetulus
- Cysteine
(chemistry, genetics)
- Eosinophils
(metabolism)
- Histamine
(immunology)
- Histamine Release
- Humans
- Lung
(immunology)
- Th2 Cells
(immunology)
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