Recombinant chimeric vaccine composed of PRRSV antigens and truncated Pseudomonas exotoxin A (PE-K13).

Abstract

A Pseudomonas exotoxin (PE-KDEL)-based chimeric subunit vaccine system was recently developed using a reverse vaccinology technique. In this study, the plasmids containing PE-PRRS chimeric subunits were constructed that composed of porcine reproductive and respiratory syndrome virus (PRRSV) antigen moieties, a ligand moiety and a Pseudomonas exotoxin A deleted domain III (PE (ΔIII)), and a carboxyl terminal moiety that includes a polypeptide with amino acid sequence KDEL (K3). The PE-PRRS combination vaccine can effectively induce not only PRRSV-specific INF-γ cellular immunity but also a slow-reacting and complement-requiring type serum neutralizing antibody in pigs. In a specific pathogen free (SPF) pig challenge model, body temperature (colonic temperature), occurrence of PRRSV viremia, nasal excretions, gross and histopathological appearances of pneumonia, and serum antibody activity (IFA and SN) titers significantly differed between the immunized group and the control group. The survey showed that a 0.3mg/dose PE-PRRS vaccine formula conferred protection against PRRSV. A field trial of PE-PRRS vaccine was performed to study the immune response of pregnant sows after vaccination in a PRRSV persist farm. The RT-PCR analysis of viremia and serological titers showed that the PE-PRRS vaccine not only increased sow reproductive performance and evoked its immune response to PRRS viremia, it also activated maternal immune protections to prevent piglets from inflicting viremia. In conclusion, we developed a novel and effective PRRS cytotoxic T-cells (CTLs)-based vaccine containing Pseudomonas exotoxin (PE-KDEL) carrier in combination with PRRSV conserved epitopes against PRRS virus.

• Authors: Hsin-Ping Yang, Tsan-Chih Wang, Shiou-Jen Wang, Shih-Ping Chen, Eva Wu, Shao-Qun Lai, Hsueh-Wei Chang, Chao-Wei Liao
• Journal: Research in veterinary science (Res Vet Sci) Vol. 95 Issue 2 Pg. 742-51 (Oct 2013) ISSN: 1532-2661 [Electronic] England
• PMID: 23712024 (Publication Type: Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Ltd. All rights reserved.

Chemical References

• Antigens, Viral
• Bacterial Toxins
• Exotoxins
• Recombinant Proteins
• Viral Vaccines
• Virulence Factors
• ADP Ribose Transferases

Topics

• ADP Ribose Transferases (genetics, metabolism)
• Animals
• Antigens, Viral
• Bacterial Toxins (genetics, metabolism)
• Exotoxins (genetics, metabolism)
• Female
• Porcine Reproductive and Respiratory Syndrome (prevention & control)
• Porcine respiratory and reproductive syndrome virus (genetics, immunology, metabolism)
• Pregnancy
• Recombinant Proteins
• Specific Pathogen-Free Organisms
• Swine
• Viral Vaccines (immunology)
• Virulence Factors (genetics, metabolism)
• Pseudomonas aeruginosa Exotoxin A