Investigation of SSEA-4 binding protein in breast cancer cells.

Abstract

SSEA-4, a sialyl-glycolipid, has been commonly used as a pluripotent human embryonic stem cell marker, and its expression is correlated with the metastasis of some malignant tumors. However, there is no in-depth functional study related to the receptor and the role of this glycolipid. Here, we report the identification of an SSEA-4-binding protein in a breast cancer cell line, MCF-7. By using affinity capture and glycan microarray techniques, the intracellular FK-506 binding protein 4 (FKBP4) was identified to bind directly to SSEA-4. The biological significance of SSEA-4/FKBP4 interaction was investigated.

• Authors: Ting-Chun Hung, Chih-Wei Lin, Tsui-Ling Hsu, Chung-Yi Wu, Chi-Huey Wong
• Journal: Journal of the American Chemical Society (J Am Chem Soc) Vol. 135 Issue 16 Pg. 5934-7 (Apr 24 2013) ISSN: 1520-5126 [Electronic] United States
• PMID: 23574147 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Polysaccharides
• Stage-Specific Embryonic Antigens
• stage-specific embryonic antigen-4
• Polyethylene Glycols
• Tacrolimus Binding Proteins
• Tacrolimus

Topics

• Breast Neoplasms (metabolism)
• Cell Line, Tumor
• Chromatography, High Pressure Liquid
• Female
• Flow Cytometry
• Humans
• Mass Spectrometry
• Microarray Analysis
• Polyethylene Glycols (chemistry)
• Polysaccharides (metabolism)
• Protein Binding
• Stage-Specific Embryonic Antigens (metabolism)
• Tacrolimus (metabolism)
• Tacrolimus Binding Proteins (metabolism)