Molecular-level dengue fever diagnostic devices made out of paper.

Abstract

This paper describes the development of nucleic acid detection in paper using a combination of commercial fluorescent probes and DNA ladders, and provides us with a better understanding of the interactions between double-stranded DNA (the amplified products in this study), fiber structures in paper, and fluorescent probes. The amplified products (the reverse-transcription and amplification of dengue virus serotype-2 RNA via RT-LAMP) in this study were subsequently fluorescently labeled in paper-based test zones (on our paper-based diagnostic device), thus fluorescent probes were used to perform the diagnosis of dengue fever, specific to serotype-2.

• Authors: Shih-Jie Lo, Shih-Chun Yang, Da-Jeng Yao, Jiann-Hwa Chen, Wu-Chun Tu, Chao-Min Cheng
• Journal: Lab on a chip (Lab Chip) Vol. 13 Issue 14 Pg. 2686-92 (Jul 21 2013) ISSN: 1473-0189 [Electronic] England
• PMID: 23563693 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Viral
• Fluorescent Dyes
• Oligonucleotide Probes
• RNA, Viral

Topics

• DNA, Viral (genetics, isolation & purification)
• Dengue (virology)
• Dengue Virus (classification, genetics, isolation & purification)
• Equipment Design
• Fluorescent Dyes (chemistry)
• Humans
• Image Processing, Computer-Assisted
• Molecular Diagnostic Techniques (instrumentation, methods)
• Nucleic Acid Amplification Techniques (instrumentation, methods)
• Oligonucleotide Probes (genetics)
• Paper
• RNA, Viral (genetics, isolation & purification)
• Reverse Transcriptase Polymerase Chain Reaction (instrumentation, methods)
• Sensitivity and Specificity