Abstract | BACKGROUND: A role for the innate immune system in driving the autoimmune T cell cascade in psoriasis has been proposed. Toll-like receptors-(TLR)-2 and -4 play a role in inflammation, atherosclerosis, and their specific role in psoriasis remains unclear. OBJECTIVE: To evaluate TLR2 and TLR4 gene expression levels in peripheral blood mononuclear cells from psoriatic patients. METHODS: Changes in TLR2 ⁄ 4 gene expressions were evaluated using quantitative real-time reverse transcription polymerase chain reaction in peripheral blood mononuclear cells, from twenty-one patients with severe psoriasis, and analysed whether there was any correlation with cytokine plasma levels (T-helper 0-, T-helper 1-, T-helper 2- or regulatory T cells-type), or Calprotectin and with S100A8 and S100A9 gene expression levels. Eleven non-psoriatic healthy controls were analysed. RESULTS: A clear increase in TLR4 gene expression was observed (3.84 ± 0.93, n = 21) together with a moderate increase in TLR2 expression (1.522 ± 0.31, n = 21). Both TLR4 and TLR2 gene expressions were significantly augmented in psoriatic patients compared with controls (all P < 0.001). Correlations between TLR2 and S100A9 gene expressions (r = 0.5145, P = 0.0170, n = 21); and between TLR2 expression and plasma interleukin-2 (r = 0.5667, P = 0.0074); interleukin-4 (r = 0.4766, P = 0.0289), interleukin-10 (r = 0.4355, P = 0.0484) and interleukin-13 (r = 0.4603, P = 0.0358), were found. When patients with atheroma plaque were considered (n = 7), both TLR4 (3.47 ± 0.99, P = 0.0156) and TLR2 (1.63 ± 0.31, P = 0.0156) expressions were significantly increased vs. controls and correlated with plasma TNF-a (r = 0.8929, P = 0.0123, in both cases). CONCLUSION: Differential TLR4 ⁄ 2 gene expressions on psoriatic peripheral blood mononuclear cells and correlations with regulatory and ⁄ or proinflammatory cytokines and ⁄ or damage-associated molecular pattern molecule S100A9 emphasize innate immune response role in psoriasis.
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Authors | S Garcia-Rodriguez, S Arias-Santiago, R Perandrés-López, L Castellote, E Zumaquero, P Navarro, A Buendía-Eisman, J-C Ruiz, J Orgaz-Molina, J Sancho, M Zubiaur |
Journal | Journal of the European Academy of Dermatology and Venereology : JEADV
(J Eur Acad Dermatol Venereol)
Vol. 27
Issue 2
Pg. 242-50
(Feb 2013)
ISSN: 1468-3083 [Electronic] England |
PMID | 23457721
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Calgranulin B
- Cytokines
- DNA Primers
- TLR2 protein, human
- TLR4 protein, human
- Toll-Like Receptor 2
- Toll-Like Receptor 4
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Topics |
- Adult
- Base Sequence
- Calgranulin B
(genetics)
- Cytokines
(blood)
- DNA Primers
- Enzyme-Linked Immunosorbent Assay
- Female
- Gene Expression
- Humans
- Male
- Middle Aged
- Monocytes
(metabolism)
- Psoriasis
(blood, genetics)
- Toll-Like Receptor 2
(genetics)
- Toll-Like Receptor 4
(genetics)
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