Abstract | BACKGROUND: An increasing body of evidence indicates that microRNAs play critical roles in androgen-independent prostate cancer ( AIPC) growth. However, the regulation of the expression of microRNAs in AIPC is not very clear. In this study, we investigated the role that the interaction between miR-200b-3p and p73 plays in the proliferation of AIPC. METHODS: We compared several relevant microRNAs and cancer related genes between the androgen-dependent prostate cancer (ADPC) cell line and the AIPC cell line using quantitative real-time PCR (Q-PCR) and Western blot. Then we examined the effect of p73 and miR-200b-3p on the proliferation of AIPC and ADPC using CCK-8. Furthermore we investigated the regulation of miR-200b-3p by p73. RESULTS: p73 and miR-200b-3p were both downregulated in the PC3 cell line ( AIPC). Down-regulation of both p73 and miR-200b-3p increased the proliferation of ADPC cells cultured with androgen-free medium, while up-regulation of p73 and miR-200b-3p decreased the proliferation of AIPC cells. When p73 was over-expressed in the AIPC cell subline, miR-200b-3p expression increased accordingly, while p73 was inhibited in ADPC cells cultured with androgen-free medium and miR-200b-3p expression decreased significantly. CONCLUSION: miR-200b-3p is down-regulated by low expression of p73 in AIPC cells, and this interaction contributes to the proliferation of AIPC.
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Authors | Minyi He, Yun Liu, Xinjun Deng, Songtao Qi, Xuegang Sun, Gang Liu, Yongguang Liu, Yawei Liu, Ming Zhao |
Journal | The Prostate
(Prostate)
Vol. 73
Issue 10
Pg. 1048-56
(Jul 2013)
ISSN: 1097-0045 [Electronic] United States |
PMID | 23389960
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 Wiley Periodicals, Inc. |
Chemical References |
- Androgens
- DNA-Binding Proteins
- MIRN200 microRNA, human
- MicroRNAs
- Nuclear Proteins
- TP73 protein, human
- Tumor Protein p73
- Tumor Suppressor Proteins
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Topics |
- Androgens
(genetics, metabolism)
- Cell Line, Tumor
- Cell Proliferation
- DNA-Binding Proteins
(genetics, metabolism)
- Down-Regulation
- Humans
- Male
- MicroRNAs
(genetics, metabolism)
- Nuclear Proteins
(genetics, metabolism)
- Prostate
(metabolism)
- Prostatic Neoplasms
(genetics, metabolism)
- Tumor Protein p73
- Tumor Suppressor Proteins
(genetics, metabolism)
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