Elevated expression of C3G protein in the peri-infarct myocardium of rats.

Abstract

BACKGROUND:

The integrin β1 subunit and its downstream molecules such as integrin-linked kinase (ILK) and focal adhesion kinase (FAK) are indispensable to the inhibition of postinfarction cardiac remodeling, ischemic cardiomyopathy, and heart failure. As a component of the integrin pathway, C3G (Crk SH3-domain-binding guanine nucleotide exchange factor) protein may also participate in postinfarction cardiac remodeling, ischemic cardiomyopathy, and heart failure.

MATERIAL/METHODS:

Experimental myocardial infarction (MI) and sham-operation (sham) models were set up in Sprague-Dawley rats. C3G protein expression in the myocardium in the sham group and in the non-infarcted myocardium of the peri-infarct zones in the MI group was examined by Western blot.

RESULTS:

The C3G protein expression in the myocardium was 0.22±0.06, n=8 in the post-sham 24-hour group; 0.29±0.10, n=8 in the post-MI 24-hour group; 0.22±0.07, n=8 in the post-sham 12-week group; and 0.56±0.14, n=8 in the post-MI 12-week group. The C3G protein expression in the myocardium in the post-MI 12-week group was significantly elevated compared to that in the post-sham 12-week group (p=0.0002), in the post-sham 24-hour group (p=0.0002), and in the post-MI 24-hour group (p=0.0006).

CONCLUSIONS:

C3G protein expression exhibits in the myocardium of rats. Furthermore, C3G protein expression is significantly elevated in the non-infarcted myocardium of the peri-infarct zones. The elevated C3G protein expression could participate in postinfarction cardiac remodeling, ischemic cardiomyopathy, and heart failure.

• Authors: Liping Wang, Gang Li, Zhihua Wang, Xiaolan Liu, Wenju Zhao
• Journal: Medical science monitor basic research (Med Sci Monit Basic Res) Vol. 19 Pg. 1-5 (Jan 01 2013) ISSN: 2325-4416 [Electronic] United States
• PMID: 23291598 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Guanine Nucleotide-Releasing Factor 2
• Collagen

Topics

• Animals
• Collagen (metabolism)
• Guanine Nucleotide-Releasing Factor 2 (metabolism)
• Immunohistochemistry
• Male
• Myocardial Infarction (metabolism, pathology, physiopathology)
• Myocardium (metabolism, pathology)
• Rats
• Rats, Sprague-Dawley