Oral antithrombotic effects of acylhydrazone derivatives.

Abstract

AIM:

In the search for new antithrombotic drug candidates, the synthesis and anti-platelet activity of a new series of N-acylhydrazones that were designed as thrombin inhibitors has been previously described. The aim of this work was to further characterize the effects of these compounds on thrombin-induced platelet aggregation and induced thrombosis in vivo.

METHODS:

In this work, four compounds were tested, LASSBio-693, 694, 743 and 752, on platelet aggregation induced by thrombin, ADP and TRAP-4A. These compounds were further tested using a mouse pulmonary thromboembolism model induced by collagen (500 µg/kg) and norepinephrine (80 µg/kg) or thrombin (2,000 UI), and a deep venous thrombosis model.

RESULTS:

At 200 µM, the compounds showed between 36% and 82% inhibition (for L-743 and L-752, respectively) of thrombin-induced platelet aggregation. The receptor agonist of PAR-4, TRAP-4A (250 µM), was used and inhibition between 43% and 77% was observed for each compound (200 µM).Compounds LASSBio-752 and 743 were the most effective in the venous thrombosis model, increasing the survival of the treated animals to 63% and 46%, respectively, in the model of collagen-induced thromboembolism and increasing to 80% (both) in the thrombin-induced model. LASSBio 743 was more effective for deep vein thrombosis, reducing the weight of the thrombus by approximately 70%.

CONCLUSION:

All compounds were administered orally and have shown effective antithrombotic action independently of the thrombotic stimulus. These results indicate that compounds LASSBio-743 and 752 are potential candidates for the treatment of cardiovascular diseases.

• Authors: Flávia Serra Frattani, Eduardo Oliveira Coriolano, Lidia Moreira Lima, Eliezer Jesus Barreiro, Russolina Benedeta Zingali
• Journal: Journal of atherosclerosis and thrombosis (J Atheroscler Thromb) Vol. 20 Issue 3 Pg. 287-95 ( 2013) ISSN: 1880-3873 [Electronic] Japan
• PMID: 23182978 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antithrombins
• Hydrazones
• Platelet Aggregation Inhibitors

Topics

• Administration, Oral
• Animals
• Antithrombins (administration & dosage, pharmacology, therapeutic use)
• Disease Models, Animal
• Hydrazones (administration & dosage, chemistry, pharmacology, therapeutic use)
• Mice
• Platelet Aggregation Inhibitors (administration & dosage, pharmacology, therapeutic use)
• Pulmonary Embolism (drug therapy)