Sensitivity of MUC5AC to topical corticosteroid is negatively associated with interleukin-17A in patients with allergic rhinitis.

Abstract

BACKGROUND:

Although Interleukin (IL)-17A has been suggested to play a role in corticosteroid hyporesponsiveness, whether IL-17A is able to affect the sensitivity of MUC5AC to intranasal corticosteroid treatment in patients with allergic rhinitis (AR) is unclear.

METHODS:

Twenty patients with moderate to severe AR were enrolled in this study and the expression of MUC5AC, IL-17A, and glucocorticoid receptor beta (GR beta) was detected using immunochemical staining and quantitative reverse transcription polymerase chain reaction (RT-PCR) before and after treatment with fluticasone propionate (FP) nasal spray for 4 weeks, respectively. In addition, the effects of FP on IL-13- and IL-17A-induced MUC5AC and GR beta were also evaluated in the primarily cultured human nasal epithelial cells (HNECs) in vitro.

RESULTS:

The increased MUC5AC expression was associated with IL-17A levels in AR, and IL-17A was found to affect the inhibition of MUC5AC by corticosteroid treatment. Both IL-13 and IL-17A significantly promoted MUC5AC mRNA expression in HNECs, and FP treatment was able to significantly inhibit MUC5AC mRNA expression in HNECs induced by IL-13 but not for that induced by IL-17A. Also, IL-17A but not IL-13 promoted GR beta mRNA expression in HNECs, which was not affected by administration of corticosteroid.

CONCLUSION:

Our results suggest that the sensitivity of MUC5AC to topical corticosteroid is negatively associated with IL-17A in AR patients. This might help us to gain more insight into the pathophysiology and the pharmacotherapeutic mechanisms on AR treatment.

• Authors: Haocheng Tang, Hongtian Wang, Jing Bai, Minghong Ding, Wenlong Liu, Wentong Xia, Qing Luo, Geng Xu, Huabin Li, Jugao Fang, Nasal Health Group and China
• Journal: American journal of rhinology & allergy (Am J Rhinol Allergy) 2012 Sep-Oct Vol. 26 Issue 5 Pg. 359-64 ISSN: 1945-8932 [Electronic] United States
• PMID: 23168149 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Androstadienes
• Biomarkers
• Interleukin-17
• MUC5AC protein, human
• Mucin 5AC
• Fluticasone

Topics

• Administration, Intranasal
• Adult
• Androstadienes (administration & dosage)
• Biomarkers (metabolism)
• Drug Resistance
• Female
• Fluticasone
• Gene Expression Regulation (drug effects, immunology)
• Humans
• Interleukin-17 (metabolism)
• Male
• Middle Aged
• Mucin 5AC (metabolism)
• Nasal Mucosa (drug effects, immunology)
• Rhinitis, Allergic, Perennial (drug therapy)
• Rhinitis, Allergic, Seasonal (drug therapy)
• Young Adult