Single-nucleotide polymorphism at CYP27B1-1260, but not VDR Taq I, is possibly associated with persistent hepatitis B virus infection.

Abstract

BACKGROUND:

Vitamin D, beyond its role in calcium and bone metabolism, exhibits immunomodulatory effects on innate and adaptive immune pathways and is suggestively related to liver diseases.

OBJECTIVE:

This study investigated the association of single-nucleotide polymorphisms in genes involved in vitamin D functions with hepatitis B virus (HBV) infection.

METHODS:

Five hundred Chinese Han subjects, including 274 chronic HBV patients, 68 HBV infection resolvers, and 158 healthy controls without HBV infection, were studied. The CYP27B1-1260 promoter and the VDR Taq I polymorphisms were genotyped by polymerase chain reaction-restriction fragment length polymorphism.

RESULTS:

Although there was no difference between HBV patients and healthy controls, HBV patients and healthy controls had a higher frequency of the CYP27B1-1260 genotype CC (15.0% vs. 2.9%, p=0.004 and 13.3% vs. 2.9%, p=0.006, respectively) and allele C (38.3% vs. 25.7%, p=0.006 and 39.2% vs. 25.7%, p=0.006, respectively) compared with resolvers. The genotype and allele frequencies of the VDR Taq I polymorphism had no difference between patients, resolvers, and healthy controls.

CONCLUSION:

These results suggest that the CYP27B1-1260 promoter polymorphism is possibly associated with the persistence, but not susceptibility to HBV infection in Chinese HBV patients, and that the VDR Taq I polymorphism is not suggested to be related to chronic HBV infection.

• Authors: Qianqian Zhu, Na Li, Qunying Han, Zhu Li, Guoyu Zhang, Fang Li, Pingping Zhang, Jinghong Chen, Yi Lv, Zhengwen Liu
• Journal: Genetic testing and molecular biomarkers (Genet Test Mol Biomarkers) Vol. 16 Issue 9 Pg. 1115-21 (Sep 2012) ISSN: 1945-0257 [Electronic] United States
• PMID: 22963605 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Receptors, Calcitriol
• 25-Hydroxyvitamin D3 1-alpha-Hydroxylase
• Taq Polymerase

Topics

• 25-Hydroxyvitamin D3 1-alpha-Hydroxylase (genetics)
• Adult
• Asian People (ethnology, genetics)
• Female
• Gene Frequency
• Genotype
• Hepatitis B, Chronic (ethnology, genetics)
• Humans
• Male
• Middle Aged
• Polymorphism, Single Nucleotide (genetics)
• Receptors, Calcitriol (genetics)
• Taq Polymerase