Abstract | OBJECTIVE: METHODS: Following complete transection at the thoracic level (T9), we immediately transplanted the grafts between the stumps of the severed spinal cords. We evaluated the functional recovery of the hindlimbs of the operated rats using the BBB locomotor rating scale system every week. Eight weeks after surgery, axonal regeneration was examined using the fluorogold (FG) retrograde tracing method. Electrophysiological analysis was carried out to evaluate the improvement in the neuronal circuits. Immunohistochemistry was employed to identify local injuries and recovery. RESULTS: The results of the Basso-Beattie-Bresnahan (BBB) scale indicated that there was no significant difference between the individual groups. The FG retrograde tracing and electrophysiological analyses indicated that the transplantation of ASN- BDNF provided a permissive environment to support neuron regeneration. CONCLUSION: The ASN- BDNF transplantation provided a promising therapeutic approach to promote axonal regeneration and recovery after SCI, and can be used as part of a combinatory treatment strategy for SCI management.
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Authors | Changyu Li, Xiangtong Zhang, Ronglong Cao, Bohai Yu, Hongsheng Liang, Min Zhou, Dayong Li, Yuehua Wang, Enzhong Liu |
Journal | PloS one
(PLoS One)
Vol. 7
Issue 8
Pg. e42813
( 2012)
ISSN: 1932-6203 [Electronic] United States |
PMID | 22952613
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Brain-Derived Neurotrophic Factor
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Topics |
- Animals
- Axons
(pathology)
- Basement Membrane
(pathology)
- Blood-Brain Barrier
- Brain-Derived Neurotrophic Factor
(metabolism)
- Electrophysiology
(methods)
- Female
- Microscopy, Electron, Scanning
(methods)
- Microscopy, Electron, Transmission
(methods)
- Nerve Regeneration
- Neurons
(metabolism)
- Rats
- Sciatic Nerve
(physiopathology, ultrastructure)
- Spinal Cord
(pathology)
- Spinal Cord Injuries
(therapy)
- Transplantation, Homologous
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