[Effect of down-regulation of histone deacetylase 2 protein expression on cell proliferation and cell cycle in cervical carcinoma].

Abstract

OBJECTIVE:

To study the effect of down-regulation of histone deacetylase 2 (HDAC2) expression on cell proliferation and cell cycle in cervical carcinoma cell lines HeLa.

METHODS:

HDAC2 siRNA and control siRNA were transfected to HeLa cells. CCK-8 and flow cytometry were used to analyze the changes of cell proliferation and cell cycle, respectively. Western blot was employed to detect the changes of cell proliferation and cell cycle-related proteins.

RESULTS:

HDAC2 siRNA significantly down-regulated the expression of HDAC2 protein in HeLa cells, resulting in marked inhibition of cell proliferation. In addition, the percentage of cells in G(0)/G(1) phase in HDAC2 siRNA group (63.3% ± 2.0%) was significantly higher than that in untreated group (29.3% ± 1.7%) or control siRNA group (29.4% ± 1.7%), F = 354.181, P = 0.000. Furthermore, Western blot demonstrated that down-regulation of HDAC2 expression decreased the expression of cyclin D1, cyclin E and CDK2 proteins but increased the expression of p21 protein.

CONCLUSIONS:

Down-regulation of HDAC2 expression mediates proliferation inhibition and cell cycle arrest. It is associated with decrease in cyclin D1, cyclin E and CDK2 protein expression and increase in p21 protein expression.

• Authors: Fang-fang Hua, Yong-hua Xia, Da-peng Wu, Rui-xiang Chen, Yu-hong Wang, Ying Pan, Jun Yang, Wu-feng Liang
• Journal: Zhonghua bing li xue za zhi = Chinese journal of pathology (Zhonghua Bing Li Xue Za Zhi) Vol. 41 Issue 7 Pg. 466-9 (Jul 2012) ISSN: 0529-5807 [Print] China
• PMID: 22932458 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CCND1 protein, human
• CCNE1 protein, human
• Cyclin E
• Oncogene Proteins
• RNA, Small Interfering
• Cyclin D1
• CDK2 protein, human
• Cyclin-Dependent Kinase 2
• HDAC2 protein, human
• Histone Deacetylase 2
• Proto-Oncogene Proteins p21(ras)

Topics

• Cell Cycle
• Cell Proliferation
• Cyclin D1 (metabolism)
• Cyclin E (metabolism)
• Cyclin-Dependent Kinase 2 (metabolism)
• Down-Regulation
• HeLa Cells
• Histone Deacetylase 2 (genetics, metabolism)
• Humans
• Oncogene Proteins (metabolism)
• Proto-Oncogene Proteins p21(ras) (metabolism)
• RNA, Small Interfering (genetics)
• Transfection