Abstract | PURPOSE: METHODS: Male C57BL/6 hTNF-α mice were fed for 6 weeks a high-fat control diet (24.50 % total fats, w/w) or high-fat diets containing either FO or KO at similar doses of n-3 PUFAs (EPA: 5.23 vs. 5.39 wt%, DHA: 2.82 vs. 2.36 wt% of total fatty acids). RESULTS: We found that KO, containing bioactive n-3 PUFAs in the form of phospholipids, was capable of modulating lipid metabolism by lowering plasma levels of TAG and cholesterol and stimulating the mitochondrial and peroxisomal fatty acid β-oxidation, as well as improving the overall carnitine turnover. Though the administration of FO was not as effective as KO in the lowering of plasma TAG, FO significantly improved the levels of all cholesterol classes in plasma. Except from the increase in the levels of IL-17 in FO-fed mice and a trend to decrease in MCP-1 levels in KO-fed animals, the levels of pro-inflammatory cytokines were not substantially different between treatment groups. CONCLUSION: Our findings demonstrate that FO and KO are comparable dietary sources of n-3 PUFAs. However, when quantitatively similar doses of n-3 PUFAs are administered, KO seems to have a greater potential to promote lipid catabolism. The effect of dietary oils on the levels of inflammatory markers in hTNF-α transgenic mice fed a high-fat diet needs further investigations.
|
Authors | Natalya Filipchuk Vigerust, Bodil Bjørndal, Pavol Bohov, Trond Brattelid, Asbjørn Svardal, Rolf Kristian Berge |
Journal | European journal of nutrition
(Eur J Nutr)
Vol. 52
Issue 4
Pg. 1315-25
(Jun 2013)
ISSN: 1436-6215 [Electronic] Germany |
PMID | 22923017
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Anti-Inflammatory Agents, Non-Steroidal
- Cytokines
- Fish Oils
- Hypolipidemic Agents
- Oils
- Phospholipids
- TNF protein, human
- Triglycerides
- Tumor Necrosis Factor-alpha
- Docosahexaenoic Acids
- Eicosapentaenoic Acid
- Carnitine
|
Topics |
- Animals
- Anti-Inflammatory Agents, Non-Steroidal
(therapeutic use)
- Carnitine
(blood, metabolism)
- Cytokines
(metabolism)
- Dietary Supplements
- Docosahexaenoic Acids
(administration & dosage, blood, metabolism, therapeutic use)
- Eicosapentaenoic Acid
(administration & dosage, blood, metabolism, therapeutic use)
- Euphausiacea
(chemistry)
- Fish Oils
(therapeutic use)
- Humans
- Hypertriglyceridemia
(blood, etiology, immunology, prevention & control)
- Hypolipidemic Agents
(therapeutic use)
- Lipid Metabolism
- Liver
(immunology, metabolism)
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Transgenic
- Oils
(therapeutic use)
- Phospholipids
(blood, metabolism)
- Triglycerides
(blood, metabolism)
- Tumor Necrosis Factor-alpha
(biosynthesis, genetics, metabolism)
|