Abstract | BACKGROUND: MATERIALS AND METHODS: In vitro, fluorescent staining, flow cytometry and Western blot were performed to analyze apoptosis and vascular endothelial growth factor ( VEGF) expression of mouse breast cancer 4T1 cells. In vivo, therapy experiments were conducted on Babl/c mice bearing breast cancer. The tumor volume and weight were measured. The number of apoptotic cells and microvascular density (MVD) in tumor tissues were assessed by TUNEL and CD34 immunostaining. Immunohistochemical assays and ELISA assay were used to detect the expression of VEGF in tissues. RESULTS: In vitro studies showed that crude fucoidan significantly decreased the viable number of 4T1 cells, induced apoptosis and down-regulated the expression of VEGF. The expression of Bcl-2 was decreased, and the ratio of Bcl-2 to Bax was significantly decreased. The expression of Survivin and phosphorylated extracellular signal regulated protein kinases (ERKs) was decreased. Cytochrome C was released from mitochondria into cytosol, and the cleaved Caspase-3 protein rose after fucoidan treatment. Intraperitoneal injection of fucoidan in breast cancer models reduced the tumor volume and weight. The enhanced antitumor efficacy was associated with decreased angiogenesis and increased induction of apoptosis. CONCLUSION:
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Authors | Meilan Xue, Yinlin Ge, Jinyu Zhang, Qing Wang, Lin Hou, Yongchao Liu, Lingling Sun, Quan Li |
Journal | PloS one
(PLoS One)
Vol. 7
Issue 8
Pg. e43483
( 2012)
ISSN: 1932-6203 [Electronic] United States |
PMID | 22916270
(Publication Type: Journal Article)
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Chemical References |
- Antineoplastic Agents
- Birc5 protein, mouse
- Inhibitor of Apoptosis Proteins
- Polysaccharides
- Proto-Oncogene Proteins c-bcl-2
- Repressor Proteins
- Survivin
- Vascular Endothelial Growth Factor A
- fucoidan
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Topics |
- Animals
- Antineoplastic Agents
(pharmacology, therapeutic use)
- Apoptosis
(drug effects)
- Blotting, Western
- Breast Neoplasms
(drug therapy, metabolism)
- Cell Line
- Enzyme-Linked Immunosorbent Assay
- Female
- Fibroblasts
(cytology, drug effects)
- Flow Cytometry
- Inhibitor of Apoptosis Proteins
(metabolism)
- Mice
- Polysaccharides
(pharmacology, therapeutic use)
- Proto-Oncogene Proteins c-bcl-2
(metabolism)
- Repressor Proteins
(metabolism)
- Survivin
- Vascular Endothelial Growth Factor A
(metabolism)
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