Abstract | BACKGROUND:
Collinin is a secondary plant metabolite belonging to the class of geranyloxycoumarins. We explored the potential beneficial impact of collinin on periodontal health by investigating its effect on Porphyromonas gingivalis (P. gingivalis), lipopolysaccharide (LPS)-induced inflammatory response of macrophages, and osteoclastogenesis. METHODS: RESULTS:
Collinin inhibited the growth of P. gingivalis. The effect was more pronounced under iron-restricted conditions. Collinin dose dependently inhibited the degradation of type I collagen by P. gingivalis. It was also a potent inhibitor of the LPS-induced inflammatory response in macrophages and completely inhibited receptor activator of nuclear factor κB ligand-dependent osteoclast differentiation and MMP-9 secretion. Last, collinin affected bone degradation mediated by mature osteoclasts by significantly decreasing the release of collagen helical peptides. CONCLUSION: Although clinical trials are required, data from these in vitro analyses support the potential of collinin as a therapeutic agent for treating inflammatory periodontitis associated with bone breakdown.
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Authors | Juliana Santos, Annie Marquis, Francesco Epifano, Salvatore Genovese, Massimo Curini, Daniel Grenier |
Journal | Journal of periodontology
(J Periodontol)
Vol. 84
Issue 5
Pg. 704-11
(May 2013)
ISSN: 1943-3670 [Electronic] United States |
PMID | 22897650
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Coumarins
- Lipopolysaccharides
- Macrophage Inflammatory Proteins
- NF-kappa B
- Plant Proteins
- RANK Ligand
- collinin
- Collagenases
- Matrix Metalloproteinase 9
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Topics |
- Bone Remodeling
(drug effects)
- Cell Differentiation
(drug effects)
- Cells, Cultured
- Collagenases
(biosynthesis, metabolism)
- Coumarins
(pharmacology)
- Humans
- Lipopolysaccharides
(antagonists & inhibitors)
- Macrophage Inflammatory Proteins
(antagonists & inhibitors)
- Matrix Metalloproteinase 9
(biosynthesis)
- NF-kappa B
(metabolism)
- Osteoclasts
(drug effects)
- Plant Proteins
(pharmacology)
- Porphyromonas gingivalis
(drug effects, metabolism)
- RANK Ligand
(antagonists & inhibitors)
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