Association of miR-146aC>G, miR-196a2T>C, and miR-499A>G polymorphisms with risk of premature ovarian failure in Korean women.

Abstract

We investigated whether microRNA (miRNA) polymorphisms (miR-146aC>G, miR-196a2T>C, and miR-499A>G) confer risk of premature ovarian failure (POF) in Korean women. DNA samples from 136 patients with POF and 234 controls were genotyped for the 3 miRNA single-nucleotide polymorphisms by polymerase chain reaction-restriction fragment length polymorphism. The miR-146aCG/miR-196a2TC combined genotype was less frequent in patients than in controls (P < .05), conferring less susceptibility. Using haplotype-based multifactor dimensionality reduction analysis, the C-C-A and G-T-A inferred haplotypes (miR-146a/miR-196a2/miR-499) were less frequent in patients, suggesting protective effects (P < .05 for each), whereas the C-T-A and G-C-A haplotypes were more frequent in patients (P < .05 for each). The C-T and G-C haplotypes (miR-146a/miR-196a2) were more frequent in patients, whereas the C-C and G-T haplotypes were less frequent in patients (P < .05 for each). However, none of the 3 miRNA polymorphisms alone was associated with POF risk. Our findings suggest that putative gene-gene interaction between miR-146 and miR-196a2 may be involved in POF development.

• Authors: HyungChul Rah, Young Joo Jeon, Sung Han Shim, Sun Hee Cha, Dong Hee Choi, Hwang Kwon, Ji Hyang Kim, Ji Eun Shin, Nam Keun Kim
• Journal: Reproductive sciences (Thousand Oaks, Calif.) (Reprod Sci) Vol. 20 Issue 1 Pg. 60-8 (Jan 2013) ISSN: 1933-7205 [Electronic] United States
• PMID: 22872486 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• MIRN146 microRNA, human
• MIRN196 microRNA, human
• MIRN499 microRNA, human
• MicroRNAs

Topics

• Adult
• Asian People (genetics)
• Female
• Genetic Association Studies (methods)
• Genetic Predisposition to Disease (genetics)
• Humans
• MicroRNAs (genetics)
• Polymorphism, Single Nucleotide (genetics)
• Primary Ovarian Insufficiency (diagnosis, epidemiology, genetics)
• Republic of Korea (epidemiology)
• Risk Factors
• Young Adult