Argocytes containing enzyme nanoparticles reduce toxic concentrations of arginine in the blood.

Abstract

A method for incorporation of arginase nanoparticles into mouse erythrocytes has been developed and the possibility of reducing blood arginine concentration in animals with experimental hyperargininemia with arginase-loaded erythrocytes (argocytes) has been studied. Argocyte infusion to animals with hyperargininemia led to a rapid decrease in blood arginine concentration within 1 h and this effect of argocytes persisted for at least 4 h. This was paralleled by an increase in plasma concentrations of urea and ornithine. Hence, plasma arginine is hydrolyzed by arginase incorporated into argocytes; argocytes are functionally active and can serve as a defense system in pathological hyperargininemia, while the method developed by us can be regarded as a new nanobiotechnology for medicine and veterinary.

• Authors: Yu G Kaminsky, E A Kosenko
• Journal: Bulletin of experimental biology and medicine (Bull Exp Biol Med) Vol. 153 Issue 3 Pg. 406-8 (Jul 2012) ISSN: 1573-8221 [Electronic] United States
• PMID: 22866323 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Arginine
• Arginase

Topics

• Animals
• Arginase (administration & dosage, chemistry, therapeutic use)
• Arginine (blood)
• Cells, Cultured
• Erythrocytes (chemistry, metabolism)
• Hyperargininemia (drug therapy)
• Mice
• Nanoparticles (chemistry)
• Nanotechnology