Optimum conditions of ultrasound-mediated destruction of bubble liposome for siRNA transfer in bladder cancer.

Abstract

BACKGROUND:

We investigated the effectiveness of ultrasound-mediated destruction of bubble liposome (UBL) for siRNA transfer by observing reduction in the luciferase activity of human bladder tumor RT-112 cells transfected with the luciferase gene (RT-112Luc) following luciferase siRNA transfer into the cells.

RESULTS:

siRNA was transferred to 26% of RT-112Luc cells by UBL and the luciferase activity of RT-112Luc cells was significantly suppressed by UBL using the luciferase siRNA, compared with that using nonspecific siRNA in vitro (p = 0.036). The luciferase activity of RT-112Luc tumor was suppressed by UBL using luciferase siRNA compared with that using nonspecific siRNA 2 days after the in vivo treatment.

CONCLUSION:

This study showed that UBL is suitable for siRNA transfer to mammalian cells.

• Authors: Suguru Fujisawa, Hiroshige Arakawa, Ryo Suzuki, Kazuo Maruyama, Tetsuya Kodama, Masahiro Yasunaga, Yoshikatsu Koga, Yasuhiro Matsumura
• Journal: Therapeutic delivery (Ther Deliv) Vol. 1 Issue 2 Pg. 247-55 (Aug 2010) ISSN: 2041-5990 [Print] England
• PMID: 22816131 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bacterial Proteins
• Liposomes
• Luminescent Proteins
• RNA, Small Interfering
• yellow fluorescent protein, Bacteria
• Luciferases, Firefly

Topics

• Animals
• Bacterial Proteins (biosynthesis, genetics)
• Cell Line, Tumor
• Female
• Genetic Therapy (methods)
• Humans
• Liposomes
• Luciferases, Firefly (biosynthesis, genetics)
• Luminescent Proteins (biosynthesis, genetics)
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• RNA Interference
• RNA, Small Interfering (metabolism)
• Time Factors
• Transfection (methods)
• Ultrasonics
• Urinary Bladder Neoplasms (genetics, metabolism, pathology, therapy)