Multifunctional role of Bcl-2 in malignant transformation and tumorigenesis of Cr(VI)-transformed lung cells.

Abstract

B-cell lymphoma-2 (Bcl-2) is an antiapoptotic protein known to be important in the regulation of apoptosis in various cell types. However, its role in malignant transformation and tumorigenesis of human lung cells is not well understood. We previously reported that chronic exposure of human lung epithelial cells to the carcinogenic hexavalent chromium Cr(VI) caused malignant transformation and Bcl-2 upregulation; however, the role of Bcl-2 in the transformation is unclear. Using a gene silencing approach, we showed that Bcl-2 plays an important role in the malignant properties of Cr(VI)-transformed cells. Downregulation of Bcl-2 inhibited the invasive and proliferative properties of the cells as well as their colony forming and angiogenic activities, which are upregulated in the transformed cells as compared to control cells. Furthermore, animal studies showed the inhibitory effect of Bcl-2 knockdown on the tumorigenesis of Cr(VI)-transformed cells. The role of Bcl-2 in malignant transformation and tumorigenesis was confirmed by gene silencing experiments using human lung carcinoma NCI-H460 cells. These cells exhibited aggressive malignant phenotypes similar to those of Cr(VI)-transformed cells. Knockdown of Bcl-2 in the H460 cells inhibited malignant and tumorigenic properties of the cells, indicating the general role of Bcl-2 in human lung tumorigenesis. Ingenuity Pathways Analysis (IPA) revealed potential effectors of Bcl-2 in tumorigenesis regulation. Additionally, using IPA together with ectopic expression of p53, we show p53 as an upstream regulator of Bcl-2 in Cr(VI)-transformed cells. Together, our results indicate the novel and multifunctional role of Bcl-2 in malignant transformation and tumorigenesis of human lung epithelial cells chronically exposed to Cr(VI).

• Authors: Djordje Medan, Sudjit Luanpitpong, Neelam Azad, Liying Wang, Bing-Hua Jiang, Mary E Davis, John B Barnett, Lan Guo, Yon Rojanasakul
• Journal: PloS one (PLoS One) Vol. 7 Issue 5 Pg. e37045 ( 2012) ISSN: 1932-6203 [Electronic] United States
• PMID: 22666341 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Proto-Oncogene Proteins c-bcl-2
• Tumor Suppressor Protein p53
• Chromium
• chromium hexavalent ion

Topics

• Animals
• Apoptosis (drug effects)
• Cell Line, Tumor
• Cell Movement (drug effects)
• Cell Proliferation (drug effects)
• Cell Transformation, Neoplastic (drug effects)
• Chromium (toxicity)
• Gene Expression Regulation, Neoplastic (drug effects)
• Gene Knockdown Techniques
• Gene Silencing
• Humans
• Lung (pathology)
• Lung Neoplasms (blood supply, genetics, metabolism, pathology)
• Male
• Mice
• Neoplasm Invasiveness
• Neovascularization, Pathologic (metabolism)
• Proto-Oncogene Proteins c-bcl-2 (deficiency, genetics, metabolism)
• Tumor Suppressor Protein p53 (metabolism)