Alterations in expression of specific microRNAs by combination of 4-HPR and EGCG inhibited growth of human malignant neuroblastoma cells.

Abstract

Malignant neuroblastomas are childhood tumors that remain mostly incurable. We explored efficacy of N-(4-hydroxyphenyl) retinamide (4-HPR) and (-)-epigallocatechin-3-gallate (EGCG) in altering expression of oncogenic microRNAs (OGmiRs) and tumor suppressor miRs (TSmiRs) for controlling growth of human malignant neuroblastoma SK-N-BE2 and IMR-32 cells. Combination of 4-HPR and EGCG most significantly decreased expression of OGmiRs (miR-92, miR-93, and miR-106b) and increased expression of TSmiRs (miR-7-1, miR-34a, and miR-99a) in both cell lines. Overexpression of miR-93 and miR-7-1, respectively, decreased and increased efficacy of treatments. Thus, alterations in expression of specific OGmiRs and TSmiRs by 4-HPR and EGCG inhibited growth of malignant neuroblastomas.

• Authors: Mrinmay Chakrabarti, Mehrab Khandkar, Naren L Banik, Swapan K Ray
• Journal: Brain research (Brain Res) Vol. 1454 Pg. 1-13 (May 15 2012) ISSN: 1872-6240 [Electronic] Netherlands
• PMID: 22498172 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: Copyright © 2012 Elsevier B.V. All rights reserved.

Chemical References

• Antineoplastic Agents
• MicroRNAs
• Fenretinide
• Catechin
• epigallocatechin gallate

Topics

• Antineoplastic Agents (pharmacology, therapeutic use)
• Apoptosis (drug effects, genetics)
• Catechin (analogs & derivatives, pharmacology, therapeutic use)
• Cell Line, Tumor
• Cell Survival (drug effects, genetics)
• Fenretinide (pharmacology, therapeutic use)
• Humans
• MicroRNAs (genetics, metabolism)
• Neuroblastoma (drug therapy, genetics, metabolism)