The anticancer activity of chloroquine-gold nanoparticles against MCF-7 breast cancer cells.
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| Abstract |
In the present study, 11-mercaptoundecanoic acid-modified gold nanoparticles (∼7 nm) were conjugated with chloroquine to explore their potential application in cancer therapeutics. The anticancer activity of chloroquine-gold nanoparticle conjugates (GNP-Chl) was demonstrated in MCF-7 breast cancer cells. The MCF-7 cells were treated with different concentrations of GNP-Chl conjugates, and the cell viability was assayed using trypan blue, resulting in an IC(50) value of 30 ± 5 μg/mL. Flow cytometry analysis revealed that the major pathway of cell death was necrosis, which was mediated by autophagy. The drug release kinetics of GNP-Chl conjugates revealed the release of chloroquine at an acidic pH, which was quantitatively estimated using optical absorbance spectroscopy. The nature of stimuli-responsive drug release and the inhibition of cancer cell growth by GNP-Chl conjugates could pave the way for the design of combinatorial therapeutic agents, particularly nanomedicine, for the treatment of cancer.
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| Authors | Prachi Joshi, Soumyananda Chakraborti, Jaime E Ramirez-Vick, Z A Ansari, Virendra Shanker, Pinak Chakrabarti, Surinder P Singh |
| Journal | Colloids and surfaces. B, Biointerfaces (Colloids Surf B Biointerfaces) Vol. 95 Pg. 195-200 (Jun 15 2012) ISSN: 1873-4367 [Electronic] Netherlands |
| PMID | 22445746 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Copyright | Copyright © 2012 Elsevier B.V. All rights reserved. |
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