Discovered on gastrointestinal stromal tumour 1 (DOG1): a useful immunohistochemical marker for diagnosing chondroblastoma.

Abstract

AIMS:

  Cellular areas of chondroblastoma are composed of polygonal chondroblasts with indented nuclei and scattered osteoclast-type multinucleated cells. To learn more about the phenotype of chondroblasts, we investigated the expression of several established immunohistochemical markers in chondroblastomas.

METHODS AND RESULTS:

  Nine chondroblastomas were analysed using immunohistochemical antibodies [CD34, α-smooth muscle actin (α-SMA), DOG1, CD117, AE1/AE3 and CD163]. Ten chondromyxoid fibromas, seven giant cell tumours of bone and four foetal proximal femurs were also analysed. The cellular areas of each chondroblastoma contained nests of DOG1(+) αSMA(+) CD117(-) CD34(-) chondroblasts, a phenotype that was not detected in chondromyxoid fibroma cases or in giant cell tumours. Although AE1/AE3 was expressed in all chondroblastomas, the staining intensity and proportion of the positive cells varied widely. Intra-lesional CD163(+) macrophages were detected in all cases of chondroblastoma, chondromyxoid fibroma and giant cell tumours.

CONCLUSIONS:

  Our results demonstrated nests of membranous DOG1(+) chondroblasts located within cellular portions of chondroblastoma containing diffuse heterogeneous infiltrates of mostly DOG1(-) chondroblasts, CD163(+) macrophages and multinucleated osteoclastic giant cells. Thus, chondroblastoma can be added to the tumours that are usually positive for DOG1, alongside gastrointestinal stromal tumour (GIST), rare solid-pseudopapillary neoplasms of the pancreas and exceptional mesenchymal tumours including uterine type retroperitoneal leiomyoma, peritoneal leiomyomatosis and synovial sarcoma.

• Authors: Hana Akpalo, Claudia Lange, Jozef Zustin
• Journal: Histopathology (Histopathology) Vol. 60 Issue 7 Pg. 1099-106 (Jun 2012) ISSN: 1365-2559 [Electronic] England
• PMID: 22335248 (Publication Type: Journal Article)
• Copyright: © 2012 Blackwell Publishing Ltd.

Chemical References

• ACTA2 protein, human
• ANO1 protein, human
• Actins
• Anion Exchange Protein 1, Erythrocyte
• Anoctamin-1
• Antiporters
• Biomarkers, Tumor
• Chloride Channels
• Neoplasm Proteins
• SLC4A3 protein, human

Topics

• Actins (metabolism)
• Adolescent
• Adult
• Anion Exchange Protein 1, Erythrocyte (metabolism)
• Anoctamin-1
• Antiporters (metabolism)
• Biomarkers, Tumor (metabolism)
• Bone Neoplasms (diagnosis, metabolism, pathology)
• Child
• Chloride Channels (metabolism)
• Chondroblastoma (diagnosis, metabolism, pathology)
• Female
• Gastrointestinal Neoplasms (metabolism)
• Gastrointestinal Stromal Tumors (metabolism)
• Humans
• Immunohistochemistry
• Male
• Neoplasm Proteins (metabolism)
• Young Adult