Protective effect of (2'S)-columbianetin from Corydalis heterocarpa on UVB-induced keratinocyte damage.

Abstract

A salt tolerant plant, Corydalis heterocarpa has been used as a folk medicine to treat travail and spasm. Recent studies have also reported antioxidant and antiinflammatory activities of compounds isolated from C. heterocarpa. In this study, the protective effect of (2'S)-columbianetin isolated from C. heterocarpa on UVB-induced human keratinocyte (HaCaT) damage was investigated. First, the appropriate energy level of UVB irradiation was determined using MTT and LDH assays. And then the protective effect of (2'S)-columbianetin on UVB induced HaCaT damage was evaluated by measuring; the changes in cell viability, LDH release level, ROS generation, cell cycle arrest and MMP expression levels. Finally, the effect of compound on MAPK and AP-1 signaling pathways were studied to understand the underlying signaling mechanisms. Result demonstrated that the presence of (2'S)-columbianetin suppressed the reactive oxygen species (ROS) generation, cell cycle arrest at sub-G1 phase and down regulation of MMP expression in UVB treated HaCaT cells. Furthermore, stress activated signaling pathways (ASK1-MAPK) and AP-1 signaling pathway were regulated by (2'S)-columbianetin treatment. These results suggest that (2'S)-columbianetin could be effectively used to protect human keratinocytes from UVB induced damage.

• Authors: Byul-Nim Ahn, Jung-Ae Kim, Chang-Suk Kong, Youngwan Seo, Se-Kwon Kim
• Journal: Journal of photochemistry and photobiology. B, Biology (J Photochem Photobiol B) Vol. 109 Pg. 20-7 (Apr 02 2012) ISSN: 1873-2682 [Electronic] Switzerland
• PMID: 22321694 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier B.V. All rights reserved.

Chemical References

• Free Radical Scavengers
• Furocoumarins
• Radiation-Protective Agents
• Tissue Inhibitor of Metalloproteinases
• Transcription Factor AP-1
• columbianetin
• Hydrogen Peroxide
• Mitogen-Activated Protein Kinases
• MAP Kinase Kinase Kinase 5
• Matrix Metalloproteinases

Topics

• Cell Cycle Checkpoints (drug effects, radiation effects)
• Cell Line
• Cell Survival (drug effects, radiation effects)
• Corydalis (chemistry)
• Enzyme Activation (drug effects, radiation effects)
• Free Radical Scavengers (isolation & purification, pharmacology)
• Furocoumarins (isolation & purification, pharmacology)
• Gene Expression Regulation, Enzymologic (drug effects, radiation effects)
• Humans
• Hydrogen Peroxide (metabolism)
• Intracellular Space (drug effects, metabolism, radiation effects)
• Keratinocytes (cytology, drug effects, metabolism, radiation effects)
• MAP Kinase Kinase Kinase 5 (metabolism)
• Matrix Metalloproteinases (genetics, metabolism)
• Mitogen-Activated Protein Kinases (metabolism)
• Phosphorylation (drug effects, radiation effects)
• Radiation-Protective Agents (isolation & purification, pharmacology)
• Tissue Inhibitor of Metalloproteinases (genetics, metabolism)
• Transcription Factor AP-1 (metabolism)
• Ultraviolet Rays (adverse effects)