Effects of hypercapnia and NO synthase inhibition in sustained hypoxic pulmonary vasoconstriction.

Abstract

BACKGROUND:

Acute respiratory disorders may lead to sustained alveolar hypoxia with hypercapnia resulting in impaired pulmonary gas exchange. Hypoxic pulmonary vasoconstriction (HPV) optimizes gas exchange during local acute (0-30 min), as well as sustained (> 30 min) hypoxia by matching blood perfusion to alveolar ventilation. Hypercapnia with acidosis improves pulmonary gas exchange in repetitive conditions of acute hypoxia by potentiating HPV and preventing pulmonary endothelial dysfunction. This study investigated, if the beneficial effects of hypercapnia with acidosis are preserved during sustained hypoxia as it occurs, e.g in permissive hypercapnic ventilation in intensive care units. Furthermore, the effects of NO synthase inhibitors under such conditions were examined.

METHOD:

We employed isolated perfused and ventilated rabbit lungs to determine the influence of hypercapnia with or without acidosis (pH corrected with sodium bicarbonate), and inhibitors of endothelial as well as inducible NO synthase on acute or sustained HPV (180 min) and endothelial permeability.

RESULTS:

In hypercapnic acidosis, HPV was intensified in sustained hypoxia, in contrast to hypercapnia without acidosis when HPV was amplified during both phases. L-NG-Nitroarginine (L-NNA), a non-selective NO synthase inhibitor, enhanced acute as well as sustained HPV under all conditions, however, the amplification of sustained HPV induced by hypercapnia with or without acidosis compared to normocapnia disappeared. In contrast 1400 W, a selective inhibitor of inducible NO synthase (iNOS), decreased HPV in normocapnia and hypercapnia without acidosis at late time points of sustained HPV and selectively reversed the amplification of sustained HPV during hypercapnia without acidosis. Hypoxic hypercapnia without acidosis increased capillary filtration coefficient (Kfc). This increase disappeared after administration of 1400 W.

CONCLUSION:

Hypercapnia with and without acidosis increased HPV during conditions of sustained hypoxia. The increase of sustained HPV and endothelial permeability in hypoxic hypercapnia without acidosis was iNOS dependent.

• Authors: Farzaneh Ketabchi, Hossein A Ghofrani, Ralph T Schermuly, Werner Seeger, Friedrich Grimminger, Bakytbek Egemnazarov, S Mostafa Shid-Moosavi, Gholam A Dehghani, Norbert Weissmann, Natascha Sommer
• Journal: Respiratory research (Respir Res) Vol. 13 Pg. 7 (Jan 31 2012) ISSN: 1465-993X [Electronic] England
• PMID: 22292558 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Enzyme Inhibitors
• Imines
• N-((3-(aminomethyl)phenyl)methyl)ethanimidamide
• Nitroarginine
• Sodium Bicarbonate
• Nitric Oxide Synthase Type II
• Nitric Oxide Synthase Type III

Topics

• Acidosis (drug therapy, physiopathology)
• Animals
• Enzyme Inhibitors (pharmacology)
• Hypercapnia (drug therapy, physiopathology)
• Hypoxia (drug therapy, physiopathology)
• Imines (pharmacology)
• Lung (blood supply, drug effects, physiopathology)
• Male
• Nitric Oxide Synthase Type II (antagonists & inhibitors, physiology)
• Nitric Oxide Synthase Type III (antagonists & inhibitors, physiology)
• Nitroarginine (pharmacology)
• Pulmonary Circulation (drug effects, physiology)
• Rabbits
• Sodium Bicarbonate (pharmacology)
• Vasoconstriction (drug effects, physiology)