[Alteration of mi-RNA expression profile after interferon treatment in HCV-infected Huh7.5.1 cells].

Abstract

OBJECTIVE:

To screen the mi-RNA expression profile after interferon treatment in cells infected with hepatitis C virus (HCV).

METHODS:

Huh-7.5.1 cells was infected with HCV by in vitro transcription and cultured with interferon. The mi-RNA microarray was used to measure the mi-RNA expression in the control group, HCV transcription group and interference group. Intra-group differences were analyzed by the 2 ((-delt delt CT)) method.

RESULTS:

With mi-RNA expressed in normal Huh-7.5.1 cells as a benchmark, expressions of 13 kinds of mi-RNAs were up-regulated after HCV infection and then down-regulated following interferon treatment; 7 were down-regulated after HCV infection and then up-regulated following interferon treatment.

CONCLUSION:

mi-RNA10a, mi-RNA21, mi-RNA149, mi-RNA152 and mi-RNA210 may be related to hepatitis C virus replication and transcription.

• Authors: Yan Lu, Xiao-kang Xing, Guang-zhong Song, Zhi Chen
• Journal: Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences (Zhejiang Da Xue Xue Bao Yi Xue Ban) Vol. 40 Issue 6 Pg. 598-602 (11 2011) ISSN: 1008-9292 [Print] China
• PMID: 22190519 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Interferon-alpha
• MicroRNAs
• RNA, Viral
• Interferons

Topics

• Cell Line, Tumor
• Gene Expression Profiling
• Hepacivirus (genetics)
• Humans
• Interferon-alpha (pharmacology)
• Interferons (pharmacology)
• MicroRNAs (drug effects, genetics)
• RNA, Viral (genetics)
• Transfection