Chondroitin sulfate (CS)
proteoglycans are strong inhibitors of structural rearrangement after
injuries of the adult CNS. In addition to CS chains,
keratan sulfate (KS) chains are also covalently attached to some
proteoglycans. CS and KS sometimes share the same core
protein, but exist as independent
sugar chains. However, the biological significance of KS remains elusive. Here, we addressed the question of whether KS is involved in plasticity after
spinal cord injury.
Keratanase II (K-II) specifically degraded
KS, i.e., not CS, in vivo. This
enzyme digestion promoted the recovery of motor and sensory function after
spinal cord injury in rats. Consistent with this, axonal regeneration/sprouting was enhanced in K-II-treated rats. K-II and the CS-degrading
enzyme chondroitinase ABC exerted comparable effects in vivo and in vitro. However, these two
enzymes worked neither additively nor synergistically. These data and further in vitro studies involving artificial
proteoglycans (KS/CS-
albumin) and heat-denatured or reduced/alkylated
proteoglycans suggested that all three components of the
proteoglycan moiety, i.e., the core
protein, CS chains, and KS chains, were required for the inhibitory activity of
proteoglycans. We conclude that KS is essential for, and has an impact comparable to that of CS on, postinjury plasticity. Our study also established that KS and CS are independent requirements for the
proteoglycan-mediated inhibition of axonal regeneration/sprouting.