Abstract |
Recent studies show that osteopontin (OPN) and its receptor cluster of differentiation 44 (CD44) are two pro-inflammatory cytokines contributing to the development of atherosclerosis. The objective of this study was to explore the inhibitory effect of kaempferol, a naturally occurring flavonoid compound, on atherogenesis and the mechanisms involved. The experiments were performed in aorta and plasma from C57BL/6J control and apolipoprotein E-deficient ( ApoE(-/-)) mice treated or not with kaempferol (50 or 100mg/kg, intragastrically) for 4 weeks. Kaempferol treatment decreased atherosclerotic lesion area, improved endothelium-dependent vasorelaxation, and increased the maximal relaxation value concomitantly with decrease in the half-maximum effective concentration, plasma OPN level, aortic OPN expression, and aortic CD44 expression in ApoE(-/-) mice. In addition, treatment with kaempferol also significantly decreased reactive oxygen species production in mice aorta. The present results suggest that kaempferol regulates OPN-CD44 pathway to inhibit the atherogenesis of ApoE(-/-) mice.
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Authors | Hong-Bo Xiao, Xiang-Yang Lu, Zhi-Liang Sun, Heng-Bo Zhang |
Journal | Toxicology and applied pharmacology
(Toxicol Appl Pharmacol)
Vol. 257
Issue 3
Pg. 405-11
(Dec 15 2011)
ISSN: 1096-0333 [Electronic] United States |
PMID | 22005275
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Elsevier Inc. All rights reserved. |
Chemical References |
- Apolipoproteins E
- Hyaluronan Receptors
- Kaempferols
- Reactive Oxygen Species
- Osteopontin
- kaempferol
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Topics |
- Animals
- Aorta
(drug effects, metabolism)
- Apolipoproteins E
(genetics)
- Atherosclerosis
(drug therapy, pathology)
- Dose-Response Relationship, Drug
- Endothelium, Vascular
(drug effects, pathology)
- Gene Expression Regulation
(drug effects)
- Hyaluronan Receptors
(drug effects, metabolism)
- Kaempferols
(administration & dosage, pharmacology)
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Osteopontin
(blood, drug effects, metabolism)
- Reactive Oxygen Species
(metabolism)
- Vasodilation
(drug effects)
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