Prospective measurement of Epstein-Barr virus-DNA in plasma and peripheral blood mononuclear cells of extranodal NK/T-cell lymphoma, nasal type.

Abstract

Epstein-Barr virus (EBV)-DNA was prospectively analyzed in plasma and mononuclear cells (MNCs) from peripheral blood in patients with extranodal natural killer (NK)/T-cell lymphoma, nasal type, to evaluate the clinical significance for diagnosis, monitoring the tumor burden, and prognostication. Thirty-three patients were enrolled, and 32 were evaluable. Pretreatment plasma and MNC EBV-DNA was detectable in 14 (range, 50-71 000 copies/mL) and 6 patients (range, 20-780 copies/μg DNA), respectively, and both were well correlated (r = 0.8741, P < .0001). Detectable plasma EBV-DNA was associated with higher clinical stage (P = .02), presence of B symptoms (P = .02), worse performance status (P = .02), and higher serum soluble IL-2 receptor level (P < .0001). Twenty-two patients attained complete response. Plasma EBV-DNA level was significantly higher in nonresponders than in responders (mean, 16,472 vs 2,645 copies/mL; P = .02). Multivariate analysis showed clinical stage (hazard ratio, 9.0; 95% confidence interval, 1.8%-45.0%) and pretreatment plasma EBV-DNA (hazard ratio, 10.6; 95% confidence interval, 1.3%-87.0%) were significant prognostic factors. Three-year overall survival of plasma EBV-DNA positive and negative patients was 42.9% and 94.4%, respectively (P = .0009). Plasma was a preferable sample for this purpose in NK/T-cell lymphoma, nasal type, and EBV-DNA level was a good indicator for response and overall survival.

• Authors: Ritsuro Suzuki, Motoko Yamaguchi, Koji Izutsu, Go Yamamoto, Kenzo Takada, Yasuaki Harabuchi, Yasushi Isobe, Hiroshi Gomyo, Tadashi Koike, Masataka Okamoto, Rie Hyo, Junji Suzumiya, Shigeo Nakamura, Keisei Kawa, Kazuo Oshimi, NK-cell Tumor Study Group
• Journal: Blood (Blood) Vol. 118 Issue 23 Pg. 6018-22 (Dec 01 2011) ISSN: 1528-0020 [Electronic] United States
• PMID: 21984805 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Viral

Topics

• Adolescent
• Adult
• Aged
• Aged, 80 and over
• DNA, Viral (blood, metabolism)
• Epstein-Barr Virus Infections (immunology, mortality, virology)
• Female
• Gene Dosage (genetics)
• Herpesvirus 4, Human (genetics)
• Humans
• Leukocytes, Mononuclear (virology)
• Lymphoma, Extranodal NK-T-Cell (immunology, mortality, virology)
• Male
• Middle Aged
• Nose Neoplasms (immunology, virology)
• Prognosis
• Proportional Hazards Models
• Prospective Studies
• Young Adult