Abstract | AIMS: This study is to elucidate whether cyclic adenosine monophosphate (cAMP)-mediated signal is involved in lower regenerative potential of cirrhotic liver. METHODS: RESULTS: The liver regeneration estimated by the rates of [(3) H]- thymidine incorporation and staining of proliferating cell nuclear antigen was significantly lower in the cirrhotic group. CREB, PKA and iPLA2 activities, assessed by western blots and electromobility shift assay, were significantly impaired after hepatectomy in the cirrhosis group. PKA and iPLA2 silencing by siRNA transfection significantly inhibited CREB activity and cell growth in transformed hepatocytes in vitro. CONCLUSIONS: CREB dysfunction, mediated by PKA and iPLA2 suppression, may be involved in the deteriorated liver regeneration in the cirrhotic rats.
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Authors | Gang Zhao, Rie Wakabayashi, Shinya Shimoda, Yumi Fukunaga, Michiaki Kumagai, Masao Tanaka, Kenji Nakano |
Journal | Hepatology research : the official journal of the Japan Society of Hepatology
(Hepatol Res)
Vol. 41
Issue 11
Pg. 1110-9
(Nov 2011)
ISSN: 1872-034X [Electronic] Netherlands |
PMID | 21955450
(Publication Type: Journal Article)
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Copyright | © 2011 The Japan Society of Hepatology. |