Abstract |
Photon emissions were measured at ambient temperature (21°C) in complete darkness once per min from cultures of 10(6) cells during the 12 h following removal from 37°C. The energy of emission was about 10(-20) J/s/cell. Of 8 different cell lines, B16-BL6 (mouse melanoma cells) demonstrated the most conspicuous emission profile. Acridine orange and ethidium bromide indicated the membranes were intact with no indication of ( trypan blue) cell necrosis. Treatments with EGF and ionomycin produced rapid early (first 3 h) increases in energy emission while glutamine-free, sodium azide and wortmanin-treated cells showed a general diminishment 3 to 9 h later. The results suggested the most probable origin of the photon emission was the plasma cell membrane. Measures from cells synchronized at the M- and S-phase supported this inference.
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Authors | Blake T Dotta, Carly A Buckner, Dianne Cameron, Robert F Lafrenie, Michael A Persinger |
Journal | General physiology and biophysics
(Gen Physiol Biophys)
Vol. 30
Issue 3
Pg. 301-9
(Sep 2011)
ISSN: 0231-5882 [Print] Slovakia |
PMID | 21952440
(Publication Type: Journal Article)
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Chemical References |
- Indoles
- Maleimides
- Epidermal Growth Factor
- Cyclosporine
- bisindolylmaleimide
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Topics |
- Animals
- Biophysics
(methods)
- Cell Culture Techniques
- Cell Cycle
- Cell Membrane
(metabolism)
- Cyclosporine
(pharmacology)
- Epidermal Growth Factor
(metabolism)
- Flow Cytometry
(methods)
- Humans
- Indoles
(pharmacology)
- Maleimides
(pharmacology)
- Melanoma, Experimental
- Mice
- Photons
- Temperature
- Time Factors
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