Immunohistochemical analysis of bone resorption regulators (RANKL and OPG), angiogenic index, and myofibroblasts in syndrome and non-syndrome odontogenic keratocysts.

Abstract

OBJECTIVE:

The aim of this study was to immunohistochemically analyse bone resorption regulators (receptor activator of nuclear factor kappa B ligand [RANKL] and osteoprotegerin [OPG]), angiogenic index, and myofibroblasts in Gorlin syndrome-related odontogenic keratocysts (SOKCs) and non-syndrome odontogenic keratocysts (NSOKCs).

STUDY DESIGN:

Twenty-two SOKCs, 22 primary NSOKCs, and eight recurrent NSOKCs were evaluated by immunohistochemistry using anti-RANKL and anti-OPG antibodies. The angiogenic index was determined by microvessel count (MVC) using anti-CD34 antibody. Anti-α-smooth muscle actin (α-SMA) antibody was used for the identification of myofibroblasts.

RESULTS:

Analysis of the expression of RANKL and OPG in the epithelial lining and fibrous capsule did not reveal significant differences between groups (P>0.05). In the epithelial lining, the RANKL/OPG ratio was RANKL<OPG and RANKL=OPG in most primary NSOCKs (54.5%) and SOKCs (59.1%), respectively (P>0.05). In the fibrous capsule, the ratio was RANKL=OPG in most primary (81.8%) and recurrent NSOKCs (75.0%) and in most SOKCs (45.5%) (P>0.05). No significant differences in the angiogenic index or number of myofibroblasts were observed between primary NSOKCs, recurrent NSOKCs, and SOKCs (P>0.05).

CONCLUSIONS:

The present results suggest that differences in the biological behaviour of SOKCs and NSOKCs may not be related to the expression of RANKL and OPG, to the RANKL/OPG ratio, to the angiogenic index, or to the number of myofibroblasts in these lesions.

• Authors: Cassiano Francisco Weege Nonaka, Roberta Barroso Cavalcante, Renato Luiz Maia Nogueira, Lélia Batista de Souza, Leão Pereira Pinto
• Journal: Archives of oral biology (Arch Oral Biol) Vol. 57 Issue 3 Pg. 230-7 (Mar 2012) ISSN: 1879-1506 [Electronic] England
• PMID: 21871606 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier Ltd. All rights reserved.

Chemical References

• Antigens, CD34
• Osteoprotegerin
• RANK Ligand

Topics

• Antigens, CD34 (analysis)
• Basal Cell Nevus Syndrome (metabolism)
• Bone Resorption (metabolism)
• Epithelial Cells (metabolism)
• Humans
• Immunohistochemistry
• Myofibroblasts (metabolism)
• Neovascularization, Pathologic
• Odontogenic Cysts (blood supply, metabolism)
• Osteoprotegerin (metabolism)
• RANK Ligand (metabolism)