[Influence of scutellarin on oxidative stress and neuronal apoptosis of rats with dementia].

Abstract

OBJECTIVE:

To investigate the influence of Scutellarin on the oxidative stress and neuronal apoptosis in rats with dementia and to reveal therapeutic mechanism of the drug to Alzheimer's disease.

METHODS:

Wistar rats were randomly divided into 5 groups: normal control group, sham group, model group,scutellarin group and positive control group. The animal model with dementia was by bilateral ventricle injection with beta-amyloid peptide (AP) 25-35 and intraperitoneal injection with D-galactose. Learning and memory ability of rats were detected by Morris Water Maze test; Histopathology of the cerebral cortex and hippocampus were observed under light microscope; Changes of Nissl's body in the hippocampus were survey by Nissl staining; The activities of SOD and MAO were detected by xanthinoxidase method and chemical method, respectively, and neuronal apoptosis was measured using flow cytometry.

RESULTS:

Compared with control group and sham group, the ability of learning and memory of the rats in model group was decreased; Neuropathological changes were observed in the hippocampus; The activity of SOD was decreased while the activity of MAO increased in brain tissues; Neuronal apoptosis percentage was increased. After treated with Scutellarin, learning and memory ability of rats with dementia was improved; The pathologic changes were alleviated; The activity of SOD was up-regulation and the activity of MAO was decreased; Neuronal apoptosis percentage was declined. No significant difference between the scutellarin group and positive drug control group was found.

CONCLUSION:

Scutellarin might play an therapeutic role by inhibiting oxidative stress and apoptosis in AD treatment.

• Authors: Li-li Guo, Jie Deng, Yong-lin Wang, Yong Huang, Zhi-zhong Guan
• Journal: Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials (Zhong Yao Cai) Vol. 34 Issue 2 Pg. 237-41 (Feb 2011) ISSN: 1001-4454 [Print] China
• PMID: 21823483 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Amyloid beta-Peptides
• Glucuronates
• Neuroprotective Agents
• Peptide Fragments
• amyloid beta-protein (25-35)
• scutellarin
• Apigenin
• Superoxide Dismutase

Topics

• Alzheimer Disease (drug therapy, pathology)
• Amyloid beta-Peptides
• Animals
• Apigenin (pharmacology, therapeutic use)
• Apoptosis (drug effects)
• Asarum (chemistry)
• Cerebral Cortex (metabolism, pathology)
• Dementia (drug therapy, metabolism, pathology)
• Disease Models, Animal
• Female
• Glucuronates (pharmacology, therapeutic use)
• Hippocampus (metabolism, pathology)
• Male
• Maze Learning (drug effects)
• Memory (drug effects)
• Neurons (metabolism, pathology)
• Neuroprotective Agents (pharmacology, therapeutic use)
• Oxidative Stress (drug effects)
• Peptide Fragments
• Random Allocation
• Rats
• Rats, Wistar
• Superoxide Dismutase (metabolism)