Type-III interferon, not type-I, is the predominant interferon induced by respiratory viruses in nasal epithelial cells.
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| Abstract |
As an innate immune response against diverse viral infections, a host induces two types of interferon (IFN), type-I (IFN-β/α) and type-III (IFN-λ). We investigated IFN inductions by respiratory viruses, including respiratory syncytial virus (RSV), measles virus and mumps virus in human nasal epithelial cells (NECs). IFN-λ, but not IFN-β/α, was induced by respiratory virus infection in primary NECs and immortalized NECs through transfection with the human telomerase reverse transcriptase gene (hTERT-NECs). In contrast, both IFN-λ and IFN-β/α were induced by RSV infection in human bronchiolar carcinoma cell line A549. Suppression of retinoic acid-inducible gene-I (RIG-I) expression using siRNA significantly reduced IFN-λ1 production in RSV-infected hTERT-NECs, while suppression of melanoma differentiation-associated gene 5 (MDA5) expression did not. Exogenous IFN-λ1 treatment suppressed RSV replication and chemokine induction in hTERT-NECs. These data indicate that IFN-λ, but not IFN-β/α, contributes to the main first line defense via RIG-I-dependent pathway against respiratory virus infection in NECs.
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| Authors | Tamaki Okabayashi, Takashi Kojima, Tomoyuki Masaki, Shin-Ichi Yokota, Tadaatsu Imaizumi, Hiroyuki Tsutsumi, Tetsuo Himi, Nobuhiro Fujii, Norimasa Sawada |
| Journal | Virus research (Virus Res) Vol. 160 Issue 1-2 Pg. 360-6 (Sep 2011) ISSN: 1872-7492 [Electronic] Netherlands |
| PMID | 21816185 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Copyright | Copyright © 2011 Elsevier B.V. All rights reserved. |
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