Cardioprotective effect of polydatin against ischemia/reperfusion injury: roles of protein kinase C and mito K(ATP) activation.

Abstract

Polydatin preconditioning (PPC) has been reported to be protective against brain and intestine ischemia/reperfusion injury (I/R injury), but whether polydatin exerts cardioprotective effect against myocardial ischemia/reperfusion and the underlying mechanisms remain unclear. Previous studies have demonstrated that oxidative stress plays an important role in the process of I/R. Elevation of oxidative agents and decline in anti-oxidant substance would promote I/R. Meanwhile, the activation of PKC signaling seems to mediate the cardioprotective effects of many drugs by alleviating Ca(2+) influx. In the present study, we reported for the first time that intravenous administration of polydatin before I/R significantly limited the infarct size, creatine phosphokinase (CPK) and lactate dehydrogenase (LDH) leakage from the damaged myocardium after I/R. The activity of SOD and the content of MDA remarkably changed in the presence of polydatin as well. However, the cardiac function-preserving and myocardial enzymes leakage-limiting effects of polydatin vanished in the presence of PKC inhibitors and mito K(ATP) channel blockers. But there was not a significant change in the activity of SOD and MDA content. We therefore conclude that PPC exerts cardioprotective effect by the activation of PKC-K(ATP)-dependent signaling and the direct anti-oxidative stress mechanisms.

• Authors: Qing Miao, Siwang Wang, Shan Miao, Jianbo Wang, Yanhua Xie, Qian Yang
• Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology (Phytomedicine) Vol. 19 Issue 1 Pg. 8-12 (Dec 15 2011) ISSN: 1618-095X [Electronic] Germany
• PMID: 21795031 (Publication Type: Journal Article)
• Copyright: Crown Copyright © 2011. Published by Elsevier GmbH. All rights reserved.

Chemical References

• Antioxidants
• Cardiotonic Agents
• Drugs, Chinese Herbal
• Enzyme Inhibitors
• Glucosides
• KATP Channels
• Stilbenes
• Malondialdehyde
• L-Lactate Dehydrogenase
• Superoxide Dismutase
• Protein Kinase C
• Creatine Kinase
• polydatin

Topics

• Animals
• Antioxidants (pharmacology)
• Cardiotonic Agents (pharmacokinetics, pharmacology)
• Creatine Kinase (metabolism)
• Disease Models, Animal
• Drugs, Chinese Herbal (pharmacology)
• Enzyme Inhibitors (pharmacology)
• Fallopia japonica (chemistry)
• Glucosides (pharmacokinetics, pharmacology)
• Injections, Intravenous
• KATP Channels (antagonists & inhibitors, metabolism)
• L-Lactate Dehydrogenase (metabolism)
• Male
• Malondialdehyde (metabolism)
• Mitochondria (metabolism)
• Myocardial Reperfusion Injury (drug therapy, enzymology, metabolism, prevention & control)
• Oxidative Stress (drug effects)
• Phytotherapy
• Protein Kinase C (antagonists & inhibitors, metabolism)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Signal Transduction
• Stilbenes (pharmacokinetics, pharmacology)
• Superoxide Dismutase (metabolism)