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Characterization of the effective cellulose degrading strain CTL-6.

Abstract
An efficient cellulose degrading bacteria exists in the thermophilic wheat straw-degrading community, WDC2. However, this strain cannot be isolated and cultured using conventional separation techniques under strict anaerobic conditions. We successfully isolated a strain of effective cellulose degrading bacteria CTL-6 using a wash, heat shock, and solid-liquid alternating process. Analysis of its properties revealed that, although the community containing the strain CTL-6 grew under aerobic conditions, the purified strain CTL-6 only grew under anaerobic culture conditions. The strain CTL-6 had a striking capability of degrading cellulose (80.9% weight loss after 9 days of culture). The highest efficiency value of the endocellulase (CMCase activity) was 0.404 micromol/(min mL), cellulose degradation efficiency by CTL-6 was remarkably high at 50-65 degrees C with the highest degradation efficiency observed at 60 degrees C. The 16S rRNA gene sequence analysis indicated that the closest relative to strain CTL-6 belonged to the genus Clostridium thermocellum. Strain CTL-6 was capable of utilizing cellulose, cellobiose, and glucose. Strain CTL-6 also grew with Sorbitol as the sole carbon source, whereas C. thermocellum is unable to do so.
AuthorsYucai Lü, Xiaofen Wang, Ning Li, Xiaojuan Wang, Masaharu Ishii, Yasuo Igarashi, Zongjun Cui
JournalJournal of environmental sciences (China) (J Environ Sci (China)) Vol. 23 Issue 4 Pg. 649-55 ( 2011) ISSN: 1001-0742 [Print] Netherlands
PMID21793408 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Bacterial Proteins
  • RNA, Ribosomal, 16S
  • Carbon
  • Cellulose
  • Cellulase
  • carboxymethylcellulase
Topics
  • Bacterial Proteins (metabolism)
  • Carbon (metabolism)
  • Cellulase (metabolism)
  • Cellulose (metabolism)
  • Clostridium (enzymology, genetics, growth & development, metabolism)
  • Denaturing Gradient Gel Electrophoresis
  • Microscopy, Phase-Contrast
  • Molecular Sequence Data
  • Paper
  • Phylogeny
  • Polymorphism, Restriction Fragment Length
  • RNA, Ribosomal, 16S (genetics)
  • Temperature

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