Abstract |
An efficient cellulose degrading bacteria exists in the thermophilic wheat straw-degrading community, WDC2. However, this strain cannot be isolated and cultured using conventional separation techniques under strict anaerobic conditions. We successfully isolated a strain of effective cellulose degrading bacteria CTL-6 using a wash, heat shock, and solid-liquid alternating process. Analysis of its properties revealed that, although the community containing the strain CTL-6 grew under aerobic conditions, the purified strain CTL-6 only grew under anaerobic culture conditions. The strain CTL-6 had a striking capability of degrading cellulose (80.9% weight loss after 9 days of culture). The highest efficiency value of the endocellulase ( CMCase activity) was 0.404 micromol/(min mL), cellulose degradation efficiency by CTL-6 was remarkably high at 50-65 degrees C with the highest degradation efficiency observed at 60 degrees C. The 16S rRNA gene sequence analysis indicated that the closest relative to strain CTL-6 belonged to the genus Clostridium thermocellum. Strain CTL-6 was capable of utilizing cellulose, cellobiose, and glucose. Strain CTL-6 also grew with Sorbitol as the sole carbon source, whereas C. thermocellum is unable to do so.
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Authors | Yucai Lü, Xiaofen Wang, Ning Li, Xiaojuan Wang, Masaharu Ishii, Yasuo Igarashi, Zongjun Cui |
Journal | Journal of environmental sciences (China)
(J Environ Sci (China))
Vol. 23
Issue 4
Pg. 649-55
( 2011)
ISSN: 1001-0742 [Print] Netherlands |
PMID | 21793408
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Bacterial Proteins
- RNA, Ribosomal, 16S
- Carbon
- Cellulose
- Cellulase
- carboxymethylcellulase
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Topics |
- Bacterial Proteins
(metabolism)
- Carbon
(metabolism)
- Cellulase
(metabolism)
- Cellulose
(metabolism)
- Clostridium
(enzymology, genetics, growth & development, metabolism)
- Denaturing Gradient Gel Electrophoresis
- Microscopy, Phase-Contrast
- Molecular Sequence Data
- Paper
- Phylogeny
- Polymorphism, Restriction Fragment Length
- RNA, Ribosomal, 16S
(genetics)
- Temperature
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