Abstract | PURPOSE: METHODS: Sixty male Sprague-Dawley rats were randomly divided into 3 groups, each containing 20 rats. Rats in the control group underwent a sham operation of the left testis. In the torsion-detorsion group, the left testis was rotated 720° for 2 hours. Rats in the treatment group received the same surgical procedure as the torsion-detorsion group, but rutin was administered intravenously at the time of detorsion. Bilateral orchiectomy was performed on half of the rats in each experimental group at 4 hours after detorsion for measurement of malondialdehyde, an indicator of intratesticular ROS content, and for evaluation of superoxide dismutase and catalase, which are endogenous antioxidant enzymes. Orchiectomy was performed on the remaining rats at 3 months after detorsion for analysis of testicular spermatogenesis. RESULTS: CONCLUSIONS:
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Authors | Si-Ming Wei, Zhi-Zhong Yan, Jian Zhou |
Journal | Journal of pediatric surgery
(J Pediatr Surg)
Vol. 46
Issue 7
Pg. 1419-24
(Jul 2011)
ISSN: 1531-5037 [Electronic] United States |
PMID | 21763845
(Publication Type: Journal Article)
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Copyright | Copyright © 2011 Elsevier Inc. All rights reserved. |
Chemical References |
- Antioxidants
- Malondialdehyde
- Rutin
- Catalase
- Superoxide Dismutase
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Topics |
- Animals
- Antioxidants
(therapeutic use)
- Catalase
(analysis)
- Drug Evaluation, Preclinical
- Male
- Malondialdehyde
(analysis)
- Orchiectomy
- Random Allocation
- Rats
- Rats, Sprague-Dawley
- Reperfusion Injury
(etiology, prevention & control)
- Rutin
(therapeutic use)
- Spermatic Cord Torsion
(complications)
- Spermatogenesis
- Superoxide Dismutase
(analysis)
- Testis
(blood supply, chemistry)
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