Biological activity of luteolin glycosides and tricin from Sasa senanensis Rehder.

Abstract

BACKGROUND:

In contrast to the several reports of alkaline extracts (Sasa-health, SE), no study of flavonoids from the leaves of S. senanensis has been reported. Four flavonoids were isolated from this plant species and their biological activities were investigated.

MATERIALS AND METHODS:

Luteolin 6-C-β-D-glucoside [1], luteolin 7-O-β-D-glucoside [2], luteolin 6-C-α-L-arabinoside [3] and tricin [4] were extracted from the leaf of S. senanensis with methanol, partitioned with ethyl acetate, separated by Sephadex LH-20 and purified by high-performance liquid chromatography (HPLC). The structure was determined by ultraviolet (UV) spectra, high-resolution mass spectra (HR-MS) and nuclear magnetic resonance (NMR).

RESULTS:

The luteolin glycosides, 1-3 showed no cytotoxicity against the human normal oral cells and oral squamous cell carcinoma cell lines used up to 0.8 mg/ml, whereas 4 was highly cytotoxic. The luteolin glycosides 1-3 protected the cells from UV induced cytotoxicity, more efficiently than 4. The anti-HIV activity of 4 (Selectivity index, SI=27) was much higher than that of the luteolin glycosides (SI=2-7), but lower than that of SE (SI=40). The scavenging activity of 1-3 against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide anion radicals was comparable with that of quercetin and, much higher than that of 4.

CONCLUSION:

The luteolin glycosides from S.senanensis show several new biological properties distinct from tricin and the anti-UV activity of the luteolin glycosides may be derived from their radical scavenging activity.

• Authors: Tomohiko Matsuta, Hiroshi Sakagami, Kazue Satoh, Taisei Kanamoto, Shigemi Terakubo, Hideki Nakashima, Madoka Kitajima, Hiroshi Oizumi, Takaaki Oizumi
• Journal: In vivo (Athens, Greece) (In Vivo) 2011 Sep-Oct Vol. 25 Issue 5 Pg. 757-62 ISSN: 1791-7549 [Electronic] Greece
• PMID: 21753130 (Publication Type: Journal Article)

Chemical References

• Anti-HIV Agents
• Cytotoxins
• Flavonoids
• Glycosides
• Radiation-Protective Agents
• tricin
• Luteolin

Topics

• Anti-HIV Agents (pharmacology)
• Cell Line, Tumor
• Cell Survival (radiation effects)
• Cytotoxins (pharmacology)
• Drug Evaluation, Preclinical
• Flavonoids (pharmacology)
• Glycosides (pharmacology)
• Humans
• Luteolin (pharmacology)
• Plant Leaves (chemistry)
• Radiation-Protective Agents (pharmacology)
• Sasa (chemistry)
• Ultraviolet Rays