Inflammatory cytokine and chemokine expression in sympathetic ophthalmia: a pilot study.

Abstract

Sympathetic ophthalmia is a bilateral uveitis that develops after penetrating injury to one eye. This study aimed to identify the inflammatory cellular sub-phenotypes and expression of pertinent inflammatory cytokines/chemokines in sympathetic ophthalmia (SO). Dalen-Fuchs nodules (DFN), granulomas, and non-granulomatous foci of inflammation were micro-dissected from 15 cases. RNA was extracted, and quantitative PCR was performed to measure IL-17, IL-18, IL-23, IFN-γ, CCL19, CXCL11, CCL17, and CCL22 transcripts. Immunohistochemical methods were used to characterize CD3, CD4, CD8, CD20, CD68, and CD163 expression. Non-granulomatous lymphocytes were predominantly CD3-positive and expressed more IFN-γ than cells within granulomas, consistent with Th1 cells. In contrast, granulomas and DFN contained mainly CD68+, CD163+/- and expressed more IL-17, IL-18, IL-23, CCL19, and CXCL11 than non-granulomatous cells. Our data indicate for the first time that M1 macrophages are the predominant inflammatory cells within granulomas and DFN of SO. We further observed high levels of IL-17 within granulomas and the presence of Th1 and M1 cells.

• Authors: Emiko Furusato, DeFen Shen, Xiaoguang Cao, Bungo Furusato, Robert B Nussenblatt, Elisabeth J Rushing, Chi-Chao Chan
• Journal: Histology and histopathology (Histol Histopathol) Vol. 26 Issue 9 Pg. 1145-51 (09 2011) ISSN: 1699-5848 [Electronic] Spain
• PMID: 21751146 (Publication Type: Journal Article, Research Support, N.I.H., Intramural)

Chemical References

• Chemokines
• Cytokines
• RNA

Topics

• Adult
• Aged
• Aged, 80 and over
• Chemokines (biosynthesis)
• Child
• Cytokines (biosynthesis)
• Eye Injuries (complications, pathology)
• Female
• Humans
• Immunohistochemistry
• Macrophages (metabolism, pathology)
• Male
• Microdissection
• Middle Aged
• Ophthalmia, Sympathetic (metabolism, pathology)
• Pilot Projects
• RNA (biosynthesis, isolation & purification)
• Reverse Transcriptase Polymerase Chain Reaction
• T-Lymphocytes (metabolism, pathology)
• Uveitis (pathology)
• Young Adult