Chaperonin containing T-complex polypeptide (CCT) subunit expression in oral mucosal wounds and fibroblasts.

Abstract

Mucosal wound healing in adults has been reported to feature diminished scar formation compared to healing skin wounds. We sought to determine if the expression pattern of chaperonin containing T-complex polypeptide (CCT) subunits in mucosal wounds and fibroblasts is different from that observed in skin wounds and fibroblasts. We found that CCT-beta is the only subunit message to be reduced in wounded mucosa versus unwounded control, and this reduction was confirmed at the protein level. In contrast, mRNA levels of CCT-zeta, -delta, -eta, and -epsilon were significantly increased in mucosal wounds. The increase in CCT-eta was also confirmed at the protein level. Expression levels of CCT-alpha, -beta, -delta; -epsilon, and -theta mRNAs were significantly increased in adult mucosal fibroblasts in culture compared to skin-derived fibroblasts. Western blot analyses confirmed a modest increase in CCT-beta in adult mucosal fibroblasts relative to skin fibroblasts, but CCT-eta protein was unaffected. These differences may contribute to the reported difference in healing outcomes between these two tissue types.

• Authors: Latha Satish, Nancy Lo, Phillip H Gallo, Sandra Johnson, Stephanie Haberman, Sandeep Kathju
• Journal: Cell stress & chaperones (Cell Stress Chaperones) Vol. 16 Issue 6 Pg. 675-80 (Nov 2011) ISSN: 1466-1268 [Electronic] Netherlands
• PMID: 21710295 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Protein Subunits
• RNA, Messenger
• Chaperonin Containing TCP-1

Topics

• Animals
• Base Sequence
• Cells, Cultured
• Chaperonin Containing TCP-1 (genetics)
• Fibroblasts (metabolism)
• Gene Expression Regulation
• Mouth Mucosa (metabolism)
• Protein Subunits (genetics)
• RNA, Messenger (genetics)
• Rabbits
• Real-Time Polymerase Chain Reaction
• Skin (metabolism)
• Wound Healing